Results 61 - 65 of 65 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 26651 | 3' | -55.3 | NC_005808.1 | + | 11914 | 0.69 | 0.433228 |
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Target: 5'- cGCgGgGUUCGCccAGGCCAaCcGCGGCg -3' miRNA: 3'- aCGaCgCAAGCG--UCUGGUaGcUGCCG- -5' |
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| 26651 | 3' | -55.3 | NC_005808.1 | + | 23443 | 0.69 | 0.442094 |
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Target: 5'- gUGCUGauuUUCaGCGGGCacgccaccguguuCAUCGGCGGCg -3' miRNA: 3'- -ACGACgc-AAG-CGUCUG-------------GUAGCUGCCG- -5' |
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| 26651 | 3' | -55.3 | NC_005808.1 | + | 12671 | 0.69 | 0.483726 |
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Target: 5'- cGCgucgGCGccaugCGCAaauUCAUCGACGGCu -3' miRNA: 3'- aCGa---CGCaa---GCGUcu-GGUAGCUGCCG- -5' |
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| 26651 | 3' | -55.3 | NC_005808.1 | + | 32681 | 0.68 | 0.488933 |
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Target: 5'- cGCgGCGUcgcggcccuugaCGCGGGCCAgcuuggucacagcgUCGGCGGUg -3' miRNA: 3'- aCGaCGCAa-----------GCGUCUGGU--------------AGCUGCCG- -5' |
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| 26651 | 3' | -55.3 | NC_005808.1 | + | 7382 | 1.12 | 0.000418 |
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Target: 5'- cUGCUGCGUUCGCAGACCAUCGACGGCg -3' miRNA: 3'- -ACGACGCAAGCGUCUGGUAGCUGCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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