Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 26654 | 5' | -52.6 | NC_005808.1 | + | 33480 | 0.67 | 0.6741 |
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Target: 5'- cGCGAUAgccaGCGGUGGCcgUGcCGCGAUaGCc -3' miRNA: 3'- -CGCUGUa---CGUCACCG--AC-GUGUUA-CGu -5' |
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| 26654 | 5' | -52.6 | NC_005808.1 | + | 33528 | 0.67 | 0.6741 |
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Target: 5'- cGCGAUAgccaGCGGUGGCcgUGcCGCGAUaGCc -3' miRNA: 3'- -CGCUGUa---CGUCACCG--AC-GUGUUA-CGu -5' |
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| 26654 | 5' | -52.6 | NC_005808.1 | + | 33576 | 0.67 | 0.6741 |
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Target: 5'- cGCGAUAgccaGCGGUGGCcgUGcCGCGAUaGCc -3' miRNA: 3'- -CGCUGUa---CGUCACCG--AC-GUGUUA-CGu -5' |
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| 26654 | 5' | -52.6 | NC_005808.1 | + | 35906 | 0.73 | 0.352576 |
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Target: 5'- -gGACAaGguGUGcCUGCACGAUGCGa -3' miRNA: 3'- cgCUGUaCguCACcGACGUGUUACGU- -5' |
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| 26654 | 5' | -52.6 | NC_005808.1 | + | 36131 | 0.77 | 0.184336 |
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Target: 5'- cGCGGCGUGCAGUucCUGCGCGAcgGCGa -3' miRNA: 3'- -CGCUGUACGUCAccGACGUGUUa-CGU- -5' |
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| 26654 | 5' | -52.6 | NC_005808.1 | + | 37029 | 0.7 | 0.513521 |
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Target: 5'- cGCGGCGcagcuUGCuGGUGGCUGgACGcgguuUGCAg -3' miRNA: 3'- -CGCUGU-----ACG-UCACCGACgUGUu----ACGU- -5' |
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| 26654 | 5' | -52.6 | NC_005808.1 | + | 38219 | 0.67 | 0.662542 |
|
Target: 5'- cGCGACG-GCGccUGGCUGCGCAuccGCc -3' miRNA: 3'- -CGCUGUaCGUc-ACCGACGUGUua-CGu -5' |
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| 26654 | 5' | -52.6 | NC_005808.1 | + | 39048 | 0.67 | 0.708458 |
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Target: 5'- uGCGGC--GCGGuUGGCgggacUGCGCGGUGUg -3' miRNA: 3'- -CGCUGuaCGUC-ACCG-----ACGUGUUACGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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