Results 61 - 66 of 66 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26656 | 3' | -60.7 | NC_005808.1 | + | 38622 | 0.66 | 0.382268 |
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Target: 5'- cCGgCCGCgGCGAGCGGcagGCCaGCGg -3' miRNA: 3'- -GCgGGUGgUGCUCGUCaagCGGgCGC- -5' |
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| 26656 | 3' | -60.7 | NC_005808.1 | + | 2211 | 0.66 | 0.391043 |
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Target: 5'- aCGCCCucgucggguagcACCACGcgcAGCAGgcggCGCguggCCGCGc -3' miRNA: 3'- -GCGGG------------UGGUGC---UCGUCaa--GCG----GGCGC- -5' |
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| 26656 | 3' | -60.7 | NC_005808.1 | + | 11601 | 0.66 | 0.391043 |
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Target: 5'- gGCUgGCCGcCGGGCAGcagUGCCaGCGg -3' miRNA: 3'- gCGGgUGGU-GCUCGUCaa-GCGGgCGC- -5' |
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| 26656 | 3' | -60.7 | NC_005808.1 | + | 29313 | 0.66 | 0.399948 |
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Target: 5'- uGCCCAgCACuucaaGGCGGUggcggUCGCCgGCc -3' miRNA: 3'- gCGGGUgGUGc----UCGUCA-----AGCGGgCGc -5' |
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| 26656 | 3' | -60.7 | NC_005808.1 | + | 38864 | 0.66 | 0.399948 |
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Target: 5'- cCGCCCucaucaaauCCGCGAGCAugagcGgaUGCgCCGCa -3' miRNA: 3'- -GCGGGu--------GGUGCUCGU-----CaaGCG-GGCGc -5' |
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| 26656 | 3' | -60.7 | NC_005808.1 | + | 26327 | 0.66 | 0.399948 |
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Target: 5'- aGCCgACCAgGccGGCGGgu-GCCCGUGc -3' miRNA: 3'- gCGGgUGGUgC--UCGUCaagCGGGCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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