Results 61 - 66 of 66 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26656 | 3' | -60.7 | NC_005808.1 | + | 2367 | 0.73 | 0.131423 |
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Target: 5'- uCGgCCugCACGuGGCAGa-CGCCCGCGu -3' miRNA: 3'- -GCgGGugGUGC-UCGUCaaGCGGGCGC- -5' |
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| 26656 | 3' | -60.7 | NC_005808.1 | + | 26137 | 0.73 | 0.125806 |
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Target: 5'- gGCCCGCCcCGAGUucgccgagcgcauggAGcgCGCCCGCc -3' miRNA: 3'- gCGGGUGGuGCUCG---------------UCaaGCGGGCGc -5' |
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| 26656 | 3' | -60.7 | NC_005808.1 | + | 32674 | 0.74 | 0.105496 |
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Target: 5'- uGCCCgacgugaaGCC-CGAGCAGUUCGCCgaCGUGc -3' miRNA: 3'- gCGGG--------UGGuGCUCGUCAAGCGG--GCGC- -5' |
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| 26656 | 3' | -60.7 | NC_005808.1 | + | 27889 | 0.76 | 0.071333 |
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Target: 5'- gGCgCCACCGCGGGCAG--CGCgCGCGa -3' miRNA: 3'- gCG-GGUGGUGCUCGUCaaGCGgGCGC- -5' |
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| 26656 | 3' | -60.7 | NC_005808.1 | + | 34264 | 0.77 | 0.069153 |
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Target: 5'- cCGCCCACgugucgggcaGCGAGCAGUUCGCgggggcgUCGCGg -3' miRNA: 3'- -GCGGGUGg---------UGCUCGUCAAGCG-------GGCGC- -5' |
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| 26656 | 3' | -60.7 | NC_005808.1 | + | 9802 | 1.09 | 0.000232 |
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Target: 5'- gCGCCCACCACGAGCAGUUCGCCCGCGg -3' miRNA: 3'- -GCGGGUGGUGCUCGUCAAGCGGGCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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