Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 2666 | 5' | -53 | NC_001491.2 | + | 81737 | 0.68 | 0.905475 |
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Target: 5'- -cGGAGCGGcCGGUGgcCACGCGcAGCa -3' miRNA: 3'- guUCUCGCC-GCUAUacGUGCGU-UCGc -5' |
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| 2666 | 5' | -53 | NC_001491.2 | + | 84370 | 1.08 | 0.005744 |
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Target: 5'- gCAAGAGCGGCGAUAUGCACGCAAGCGc -3' miRNA: 3'- -GUUCUCGCCGCUAUACGUGCGUUCGC- -5' |
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| 2666 | 5' | -53 | NC_001491.2 | + | 85585 | 0.75 | 0.589671 |
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Target: 5'- aCAGGAGCGGCGAga-GCaaagGCGCAAGa- -3' miRNA: 3'- -GUUCUCGCCGCUauaCG----UGCGUUCgc -5' |
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| 2666 | 5' | -53 | NC_001491.2 | + | 101817 | 0.68 | 0.929012 |
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Target: 5'- --uGAGCGGCGGcAUGCcccACGCAucAGUc -3' miRNA: 3'- guuCUCGCCGCUaUACG---UGCGU--UCGc -5' |
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| 2666 | 5' | -53 | NC_001491.2 | + | 119878 | 0.72 | 0.761425 |
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Target: 5'- aGAGAGCGGCGuaAUGUGgACGCuguuugacgacacAGCGu -3' miRNA: 3'- gUUCUCGCCGC--UAUACgUGCGu------------UCGC- -5' |
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| 2666 | 5' | -53 | NC_001491.2 | + | 135404 | 0.69 | 0.892209 |
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Target: 5'- aAAGAGCGGgGucgccggGUGCGCGUccGAGUa -3' miRNA: 3'- gUUCUCGCCgCua-----UACGUGCG--UUCGc -5' |
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| 2666 | 5' | -53 | NC_001491.2 | + | 137739 | 0.72 | 0.755528 |
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Target: 5'- gCAAGA-CGGCGcgGUAUGcCugGCAGGCa -3' miRNA: 3'- -GUUCUcGCCGC--UAUAC-GugCGUUCGc -5' |
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| 2666 | 5' | -53 | NC_001491.2 | + | 138013 | 0.68 | 0.911737 |
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Target: 5'- uGAGuGUGGUGAgagAUGgagaGCGCGAGCa -3' miRNA: 3'- gUUCuCGCCGCUa--UACg---UGCGUUCGc -5' |
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| 2666 | 5' | -53 | NC_001491.2 | + | 139876 | 0.67 | 0.948509 |
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Target: 5'- gGAGAGCucGGagGGUGUGUACGCuauAAGCu -3' miRNA: 3'- gUUCUCG--CCg-CUAUACGUGCG---UUCGc -5' |
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| 2666 | 5' | -53 | NC_001491.2 | + | 147184 | 0.72 | 0.765332 |
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Target: 5'- -cAGGGCGGCGA---GCGacgGCGAGCGa -3' miRNA: 3'- guUCUCGCCGCUauaCGUg--CGUUCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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