Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 2666 | 5' | -53 | NC_001491.2 | + | 26698 | 0.66 | 0.960542 |
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Target: 5'- gGAGcuuGCGGCcgcggGCGCGCAGGUa -3' miRNA: 3'- gUUCu--CGCCGcuauaCGUGCGUUCGc -5' |
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| 2666 | 5' | -53 | NC_001491.2 | + | 12486 | 0.66 | 0.956771 |
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Target: 5'- --cGGGCGGCGGgc-GCcCGCGGGUc -3' miRNA: 3'- guuCUCGCCGCUauaCGuGCGUUCGc -5' |
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| 2666 | 5' | -53 | NC_001491.2 | + | 139876 | 0.67 | 0.948509 |
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Target: 5'- gGAGAGCucGGagGGUGUGUACGCuauAAGCu -3' miRNA: 3'- gUUCUCG--CCg-CUAUACGUGCG---UUCGc -5' |
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| 2666 | 5' | -53 | NC_001491.2 | + | 4935 | 0.67 | 0.948509 |
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Target: 5'- aCGGGGuuuucGCGGCGuucugcgaGUGC-CGCGGGCGa -3' miRNA: 3'- -GUUCU-----CGCCGCua------UACGuGCGUUCGC- -5' |
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| 2666 | 5' | -53 | NC_001491.2 | + | 29946 | 0.67 | 0.948509 |
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Target: 5'- gCAuGGGgGGgGGUGUGgACGUggGUGg -3' miRNA: 3'- -GUuCUCgCCgCUAUACgUGCGuuCGC- -5' |
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| 2666 | 5' | -53 | NC_001491.2 | + | 32634 | 0.67 | 0.94401 |
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Target: 5'- gCGGGAGCGuCGAa----GCGCAAGCGg -3' miRNA: 3'- -GUUCUCGCcGCUauacgUGCGUUCGC- -5' |
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| 2666 | 5' | -53 | NC_001491.2 | + | 74112 | 0.68 | 0.929012 |
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Target: 5'- cCGAGuAGUGGCcaauUAUGUGCGCA-GCGa -3' miRNA: 3'- -GUUC-UCGCCGcu--AUACGUGCGUuCGC- -5' |
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| 2666 | 5' | -53 | NC_001491.2 | + | 64789 | 0.68 | 0.929012 |
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Target: 5'- uGAGAGuCGGaCGcGUGUGCccCGCGGGCa -3' miRNA: 3'- gUUCUC-GCC-GC-UAUACGu-GCGUUCGc -5' |
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| 2666 | 5' | -53 | NC_001491.2 | + | 101817 | 0.68 | 0.929012 |
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Target: 5'- --uGAGCGGCGGcAUGCcccACGCAucAGUc -3' miRNA: 3'- guuCUCGCCGCUaUACG---UGCGU--UCGc -5' |
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| 2666 | 5' | -53 | NC_001491.2 | + | 84370 | 1.08 | 0.005744 |
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Target: 5'- gCAAGAGCGGCGAUAUGCACGCAAGCGc -3' miRNA: 3'- -GUUCUCGCCGCUAUACGUGCGUUCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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