Results 1 - 20 of 20 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26662 | 3' | -66 | NC_005808.1 | + | 29426 | 0.66 | 0.193894 |
Target: 5'- gCuGGGGCaAGGCcaccGGCGCGugguucgaggacuuGGCCGGc- -3' miRNA: 3'- gGuCCCCG-UCCG----UCGCGC--------------CCGGCCaa -5' |
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26662 | 3' | -66 | NC_005808.1 | + | 37237 | 0.66 | 0.185573 |
Target: 5'- uCCAcGGcGCGgauGGCGGCGUGGGCgaGGUc -3' miRNA: 3'- -GGUcCC-CGU---CCGUCGCGCCCGg-CCAa -5' |
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26662 | 3' | -66 | NC_005808.1 | + | 31018 | 0.66 | 0.171655 |
Target: 5'- uCCAcGGGCuugccggccgAGGCAaucGCGCGGGCCa--- -3' miRNA: 3'- -GGUcCCCG----------UCCGU---CGCGCCCGGccaa -5' |
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26662 | 3' | -66 | NC_005808.1 | + | 36045 | 0.66 | 0.171207 |
Target: 5'- aCCAGcGGGCGcguGGCGcugcGCGCGGacacguacagguuGCCGGg- -3' miRNA: 3'- -GGUC-CCCGU---CCGU----CGCGCC-------------CGGCCaa -5' |
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26662 | 3' | -66 | NC_005808.1 | + | 10937 | 0.66 | 0.167227 |
Target: 5'- gCAGGGccuCGGGCAguuGCGCGGcgacaGCCGGg- -3' miRNA: 3'- gGUCCCc--GUCCGU---CGCGCC-----CGGCCaa -5' |
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26662 | 3' | -66 | NC_005808.1 | + | 25600 | 0.67 | 0.164619 |
Target: 5'- aCGGGGGCGcugccggcGGCGGCaaaaccgaccucaucGCGGGCCuGa- -3' miRNA: 3'- gGUCCCCGU--------CCGUCG---------------CGCCCGGcCaa -5' |
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26662 | 3' | -66 | NC_005808.1 | + | 31075 | 0.67 | 0.15619 |
Target: 5'- aUCAGGGGCcuGGUgccgcugcucaccucGGUGCaGGCCGGc- -3' miRNA: 3'- -GGUCCCCGu-CCG---------------UCGCGcCCGGCCaa -5' |
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26662 | 3' | -66 | NC_005808.1 | + | 6157 | 0.67 | 0.154144 |
Target: 5'- cCCAGGGGUgAGGCacgGGCacccuuaGCGGGCCu--- -3' miRNA: 3'- -GGUCCCCG-UCCG---UCG-------CGCCCGGccaa -5' |
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26662 | 3' | -66 | NC_005808.1 | + | 22765 | 0.67 | 0.142753 |
Target: 5'- cCCAGGGuGaGGGCcgauuGGUcaacgcccuGCGGGCCGGUc -3' miRNA: 3'- -GGUCCC-CgUCCG-----UCG---------CGCCCGGCCAa -5' |
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26662 | 3' | -66 | NC_005808.1 | + | 2221 | 0.67 | 0.141619 |
Target: 5'- --cGGGuagcaccacgcgcaGCAGGCGGCGCGuGGCCGc-- -3' miRNA: 3'- gguCCC--------------CGUCCGUCGCGC-CCGGCcaa -5' |
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26662 | 3' | -66 | NC_005808.1 | + | 23650 | 0.68 | 0.131783 |
Target: 5'- gCCGGcGGCGGGCAGCaacugcaccagGCgacccucggucGGGCCGGa- -3' miRNA: 3'- -GGUCcCCGUCCGUCG-----------CG-----------CCCGGCCaa -5' |
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26662 | 3' | -66 | NC_005808.1 | + | 6453 | 0.68 | 0.13073 |
Target: 5'- aCCGGGccGGCcGGCcuGCGCcugauugccgacguGGGCCGGUUc -3' miRNA: 3'- -GGUCC--CCGuCCGu-CGCG--------------CCCGGCCAA- -5' |
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26662 | 3' | -66 | NC_005808.1 | + | 28075 | 0.68 | 0.128303 |
Target: 5'- aCAGcGGGCAGGUGGcCGCGGcGUCGu-- -3' miRNA: 3'- gGUC-CCCGUCCGUC-GCGCC-CGGCcaa -5' |
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26662 | 3' | -66 | NC_005808.1 | + | 17682 | 0.68 | 0.118366 |
Target: 5'- gCuGGGcGUAGGCgaAGCGCcacucGGGCCGGg- -3' miRNA: 3'- gGuCCC-CGUCCG--UCGCG-----CCCGGCCaa -5' |
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26662 | 3' | -66 | NC_005808.1 | + | 19621 | 0.69 | 0.108854 |
Target: 5'- uCCGGGGucauGCGGGCcgcgacaucggccAGCGuCGGGuuGGUg -3' miRNA: 3'- -GGUCCC----CGUCCG-------------UCGC-GCCCggCCAa -5' |
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26662 | 3' | -66 | NC_005808.1 | + | 675 | 0.69 | 0.10623 |
Target: 5'- aCCAGGccGCcuacGGCGGCGCcGGCCGGg- -3' miRNA: 3'- -GGUCCc-CGu---CCGUCGCGcCCGGCCaa -5' |
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26662 | 3' | -66 | NC_005808.1 | + | 41602 | 0.7 | 0.089217 |
Target: 5'- gCCAGuGGcGCGGGCAGCGggcugacggugaaGGGCUGGc- -3' miRNA: 3'- -GGUC-CC-CGUCCGUCGCg------------CCCGGCCaa -5' |
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26662 | 3' | -66 | NC_005808.1 | + | 1360 | 0.71 | 0.074402 |
Target: 5'- gCCcuGGGCGcGGCGGUGUGGGCgGGc- -3' miRNA: 3'- -GGucCCCGU-CCGUCGCGCCCGgCCaa -5' |
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26662 | 3' | -66 | NC_005808.1 | + | 22486 | 0.73 | 0.049013 |
Target: 5'- aCCAGGGGCGGGC----UGGGCUGGUc -3' miRNA: 3'- -GGUCCCCGUCCGucgcGCCCGGCCAa -5' |
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26662 | 3' | -66 | NC_005808.1 | + | 12266 | 1.06 | 0.000105 |
Target: 5'- aCCAGGGGCAGGCAGCGCGGGCCGGUUu -3' miRNA: 3'- -GGUCCCCGUCCGUCGCGCCCGGCCAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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