Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 26666 | 5' | -55.8 | NC_005808.1 | + | 19634 | 0.66 | 0.584696 |
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Target: 5'- gGGCCgcGACAUCGGccaGCGUcgGGUUGguGGu -3' miRNA: 3'- -CCGG--CUGUAGCUc--CGCG--UCAACguCU- -5' |
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| 26666 | 5' | -55.8 | NC_005808.1 | + | 8127 | 0.66 | 0.584696 |
|
Target: 5'- cGGCCGGCGUUGguuguGGGCGU----GCGGGu -3' miRNA: 3'- -CCGGCUGUAGC-----UCCGCGucaaCGUCU- -5' |
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| 26666 | 5' | -55.8 | NC_005808.1 | + | 17449 | 0.66 | 0.584696 |
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Target: 5'- cGCCaGCAUCGAGGC-CAGgUGCc-- -3' miRNA: 3'- cCGGcUGUAGCUCCGcGUCaACGucu -5' |
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| 26666 | 5' | -55.8 | NC_005808.1 | + | 36522 | 0.66 | 0.595806 |
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Target: 5'- cGCuCGACGacuUCGAGGCGguGUgaugaGCAc- -3' miRNA: 3'- cCG-GCUGU---AGCUCCGCguCAa----CGUcu -5' |
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| 26666 | 5' | -55.8 | NC_005808.1 | + | 23330 | 0.66 | 0.63376 |
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Target: 5'- cGCCGACAccgaccugaccaugcUguuccccagCGAGGCGCGGUcGguGGc -3' miRNA: 3'- cCGGCUGU---------------A---------GCUCCGCGUCAaCguCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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