Results 41 - 45 of 45 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26666 | 5' | -55.8 | NC_005808.1 | + | 37341 | 0.66 | 0.629288 |
Target: 5'- aGGCCGcgaGCA-CGuGGCGCAGcaugGCAu- -3' miRNA: 3'- -CCGGC---UGUaGCuCCGCGUCaa--CGUcu -5' |
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26666 | 5' | -55.8 | NC_005808.1 | + | 37413 | 0.66 | 0.629288 |
Target: 5'- cGGCCGGCAggUCGGGGUcgGCAa-UGCGu- -3' miRNA: 3'- -CCGGCUGU--AGCUCCG--CGUcaACGUcu -5' |
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26666 | 5' | -55.8 | NC_005808.1 | + | 39331 | 0.69 | 0.436862 |
Target: 5'- gGGCCGACuAUCu-GGCGCGGaaGCuGAa -3' miRNA: 3'- -CCGGCUG-UAGcuCCGCGUCaaCGuCU- -5' |
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26666 | 5' | -55.8 | NC_005808.1 | + | 39890 | 0.67 | 0.573628 |
Target: 5'- uGCCGACAccUCGAcguuGCGCAGgUGCGc- -3' miRNA: 3'- cCGGCUGU--AGCUc---CGCGUCaACGUcu -5' |
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26666 | 5' | -55.8 | NC_005808.1 | + | 40299 | 0.66 | 0.629288 |
Target: 5'- gGGCaaaCGGCAacgguUCGAGGCGCAcGgcaugGCGGu -3' miRNA: 3'- -CCG---GCUGU-----AGCUCCGCGU-Caa---CGUCu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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