Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 26666 | 5' | -55.8 | NC_005808.1 | + | 4172 | 0.74 | 0.223356 |
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Target: 5'- cGCCGugGUCGAGGUGUAGccaGUGGGc -3' miRNA: 3'- cCGGCugUAGCUCCGCGUCaa-CGUCU- -5' |
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| 26666 | 5' | -55.8 | NC_005808.1 | + | 2436 | 0.7 | 0.362562 |
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Target: 5'- aGGCCGGCcUCGAagaucGGCGCcacgauGUUGCAc- -3' miRNA: 3'- -CCGGCUGuAGCU-----CCGCGu-----CAACGUcu -5' |
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| 26666 | 5' | -55.8 | NC_005808.1 | + | 1329 | 0.68 | 0.456766 |
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Target: 5'- aGGCCGACAagcgCGAGGaagcCGUGGUcgGCGGc -3' miRNA: 3'- -CCGGCUGUa---GCUCC----GCGUCAa-CGUCu -5' |
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| 26666 | 5' | -55.8 | NC_005808.1 | + | 885 | 0.66 | 0.584696 |
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Target: 5'- uGGCCGACGuUCGgcaagggcuGGGCGCGGc-GCGu- -3' miRNA: 3'- -CCGGCUGU-AGC---------UCCGCGUCaaCGUcu -5' |
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| 26666 | 5' | -55.8 | NC_005808.1 | + | 829 | 0.66 | 0.618112 |
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Target: 5'- uGCCGaACGUCGgccAGGUggGCAGguuggUGUAGAa -3' miRNA: 3'- cCGGC-UGUAGC---UCCG--CGUCa----ACGUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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