Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2667 | 3' | -48.2 | NC_001491.2 | + | 80157 | 0.66 | 0.999487 |
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Target: 5'- uAACuAUUGUUACUUGCcgccugaccGGCGCC-CCg -3' miRNA: 3'- cUUGuUAGCAGUGAAUG---------CUGCGGuGG- -5' |
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| 2667 | 3' | -48.2 | NC_001491.2 | + | 109392 | 0.66 | 0.999487 |
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Target: 5'- -uACGGcuUCGUUguAUUUugGGCGuCCGCCg -3' miRNA: 3'- cuUGUU--AGCAG--UGAAugCUGC-GGUGG- -5' |
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| 2667 | 3' | -48.2 | NC_001491.2 | + | 81495 | 0.66 | 0.99959 |
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Target: 5'- -uGCAG-CGgagaGCgc-CGACGCCGCCa -3' miRNA: 3'- cuUGUUaGCag--UGaauGCUGCGGUGG- -5' |
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| 2667 | 3' | -48.2 | NC_001491.2 | + | 149706 | 0.66 | 0.999667 |
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Target: 5'- aGGACAAUCGcauaaccgcugcaUCACcggaguggGCGugGCCucCCa -3' miRNA: 3'- -CUUGUUAGC-------------AGUGaa------UGCugCGGu-GG- -5' |
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| 2667 | 3' | -48.2 | NC_001491.2 | + | 83392 | 0.66 | 0.999704 |
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Target: 5'- uGAGCAAcuauuugccgucagCGUCGCUgucuuccCGGCGCCuuggGCCg -3' miRNA: 3'- -CUUGUUa-------------GCAGUGAau-----GCUGCGG----UGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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