miRNA: 3'- cuugUUAGCaGUGaa--UGCUG-CGGUGG- -5'
miRNA: 3'- cUUGUUAGC-AGUGAa--UGCUGCGgUGG- -5'
miRNA: 3'- cuUGUUAGCaGUGAA--UgCUGCGgUGG- -5'
miRNA: 3'- -CUUGU--UAGC---AGUGAAUGCUgCGGUGg -5'
miRNA: 3'- -CUUGUUAGCAGUGAAUGCUGCGGUGG- -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2667 | 3' | -48.2 | NC_001491.2 | + | 120274 | 0.71 | 0.981478 |
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Target: 5'- ---gGGUCG-CGCggcgGCGACuGCCACCa -3' miRNA: 3'- cuugUUAGCaGUGaa--UGCUG-CGGUGG- -5' |
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| 2667 | 3' | -48.2 | NC_001491.2 | + | 141579 | 0.72 | 0.968183 |
|
Target: 5'- uGACGAUUGcUCGCUggcAUGGCGCgACCu -3' miRNA: 3'- cUUGUUAGC-AGUGAa--UGCUGCGgUGG- -5' |
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| 2667 | 3' | -48.2 | NC_001491.2 | + | 83608 | 0.72 | 0.957533 |
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Target: 5'- -cACAAUCG-CGCUUggGgGACGCgGCCa -3' miRNA: 3'- cuUGUUAGCaGUGAA--UgCUGCGgUGG- -5' |
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| 2667 | 3' | -48.2 | NC_001491.2 | + | 128534 | 0.74 | 0.911964 |
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Target: 5'- cGAGCAucAUCGcagUUACUUGCGGgGCCGCg -3' miRNA: 3'- -CUUGU--UAGC---AGUGAAUGCUgCGGUGg -5' |
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| 2667 | 3' | -48.2 | NC_001491.2 | + | 83505 | 1.12 | 0.011645 |
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Target: 5'- gGAACAAUCGUCACUUACGACGCCACCc -3' miRNA: 3'- -CUUGUUAGCAGUGAAUGCUGCGGUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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