Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position![]() |
R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2667 | 3' | -48.2 | NC_001491.2 | + | 18769 | 0.68 | 0.996515 |
Target: 5'- aGGCAuaaaAUUGUCACUguuaGCGugGUCGCa -3' miRNA: 3'- cUUGU----UAGCAGUGAa---UGCugCGGUGg -5' |
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2667 | 3' | -48.2 | NC_001491.2 | + | 12546 | 0.69 | 0.993503 |
Target: 5'- cGAGCGGgcgCGggaGCgcGCGAgCGCCGCCu -3' miRNA: 3'- -CUUGUUa--GCag-UGaaUGCU-GCGGUGG- -5' |
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2667 | 3' | -48.2 | NC_001491.2 | + | 3964 | 0.68 | 0.997923 |
Target: 5'- -uGCGAUCGUaGCg-GCGGCucaugGCCACCa -3' miRNA: 3'- cuUGUUAGCAgUGaaUGCUG-----CGGUGG- -5' |
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2667 | 3' | -48.2 | NC_001491.2 | + | 3043 | 0.67 | 0.999032 |
Target: 5'- uGGCGGUCGUaGCcc-CGGCGCCuCCg -3' miRNA: 3'- cUUGUUAGCAgUGaauGCUGCGGuGG- -5' |
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2667 | 3' | -48.2 | NC_001491.2 | + | 68 | 0.69 | 0.993503 |
Target: 5'- ------aCGUCACUUccugUGACGUCACCg -3' miRNA: 3'- cuuguuaGCAGUGAAu---GCUGCGGUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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