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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26670 | 3' | -52.6 | NC_005808.1 | + | 2318 | 0.66 | 0.799115 |
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Target: 5'- gUCCAGCGacaggcacagaucgcGCAgCCUGuCCUCGaUCUGu -3' miRNA: 3'- -AGGUUGC---------------UGUaGGACuGGAGCaAGGC- -5' |
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| 26670 | 3' | -52.6 | NC_005808.1 | + | 23872 | 0.67 | 0.774825 |
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Target: 5'- gUUCGACGGCAUCCcGugCcgcCGUaCCGa -3' miRNA: 3'- -AGGUUGCUGUAGGaCugGa--GCAaGGC- -5' |
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| 26670 | 3' | -52.6 | NC_005808.1 | + | 13506 | 0.67 | 0.743221 |
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Target: 5'- gUCCcGC-ACGggCCUGACCUCGgcgcCCGa -3' miRNA: 3'- -AGGuUGcUGUa-GGACUGGAGCaa--GGC- -5' |
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| 26670 | 3' | -52.6 | NC_005808.1 | + | 26009 | 0.67 | 0.743221 |
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Target: 5'- aCCAucGCGugGUC--GGCCUCG-UCCGg -3' miRNA: 3'- aGGU--UGCugUAGgaCUGGAGCaAGGC- -5' |
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| 26670 | 3' | -52.6 | NC_005808.1 | + | 12619 | 0.68 | 0.699481 |
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Target: 5'- gUUCGACGgcuGCAUCCaGGCCgUCGUgcgCCGc -3' miRNA: 3'- -AGGUUGC---UGUAGGaCUGG-AGCAa--GGC- -5' |
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| 26670 | 3' | -52.6 | NC_005808.1 | + | 27815 | 0.68 | 0.699481 |
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Target: 5'- uUCCAGCGACAUCgUGAagcagaUCGacaCCGa -3' miRNA: 3'- -AGGUUGCUGUAGgACUgg----AGCaa-GGC- -5' |
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| 26670 | 3' | -52.6 | NC_005808.1 | + | 1371 | 0.7 | 0.552342 |
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Target: 5'- aUCCGGCGACcgaagcgcaaGUCCUcgcggaauucuucGACCU-GUUCCGg -3' miRNA: 3'- -AGGUUGCUG----------UAGGA-------------CUGGAgCAAGGC- -5' |
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| 26670 | 3' | -52.6 | NC_005808.1 | + | 28575 | 1.09 | 0.001477 |
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Target: 5'- uUCCAACGACAUCCUGACCUCGUUCCGu -3' miRNA: 3'- -AGGUUGCUGUAGGACUGGAGCAAGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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