Results 1 - 20 of 21 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26671 | 5' | -57.7 | NC_005808.1 | + | 33315 | 0.66 | 0.475201 |
Target: 5'- aGUCAA--UGCGCAgCCGGUGuaCCGGGc -3' miRNA: 3'- aUAGUUagACGCGU-GGCCGC--GGCCUu -5' |
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26671 | 5' | -57.7 | NC_005808.1 | + | 21600 | 0.66 | 0.464888 |
Target: 5'- gGUC-AUCUGCGgAUUGGUGCCGu-- -3' miRNA: 3'- aUAGuUAGACGCgUGGCCGCGGCcuu -5' |
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26671 | 5' | -57.7 | NC_005808.1 | + | 30050 | 0.66 | 0.454691 |
Target: 5'- --cCAcgUauuCGCACUGGUGCCGGAAa -3' miRNA: 3'- auaGUuaGac-GCGUGGCCGCGGCCUU- -5' |
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26671 | 5' | -57.7 | NC_005808.1 | + | 19929 | 0.66 | 0.454691 |
Target: 5'- --gCAA-CUGCGCACCGaGCGCgaaagcUGGAu -3' miRNA: 3'- auaGUUaGACGCGUGGC-CGCG------GCCUu -5' |
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26671 | 5' | -57.7 | NC_005808.1 | + | 25309 | 0.66 | 0.444618 |
Target: 5'- -------aUGCGCGCCcgcGGCGCCGGc- -3' miRNA: 3'- auaguuagACGCGUGG---CCGCGGCCuu -5' |
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26671 | 5' | -57.7 | NC_005808.1 | + | 12603 | 0.66 | 0.43467 |
Target: 5'- gAUgAAUUUGCGCA-UGGCGCCGa-- -3' miRNA: 3'- aUAgUUAGACGCGUgGCCGCGGCcuu -5' |
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26671 | 5' | -57.7 | NC_005808.1 | + | 12989 | 0.67 | 0.415169 |
Target: 5'- --------aGCGC-CCGGCGCUGGAc -3' miRNA: 3'- auaguuagaCGCGuGGCCGCGGCCUu -5' |
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26671 | 5' | -57.7 | NC_005808.1 | + | 18615 | 0.67 | 0.415169 |
Target: 5'- --cCAGUCUGUGCGCCGaauacGCGCauGAAu -3' miRNA: 3'- auaGUUAGACGCGUGGC-----CGCGgcCUU- -5' |
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26671 | 5' | -57.7 | NC_005808.1 | + | 7983 | 0.67 | 0.396214 |
Target: 5'- uUGUCAggCaGCaGCACCacGGCGUCGGAc -3' miRNA: 3'- -AUAGUuaGaCG-CGUGG--CCGCGGCCUu -5' |
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26671 | 5' | -57.7 | NC_005808.1 | + | 7554 | 0.67 | 0.386948 |
Target: 5'- -cUCGAUCUGCGgcgcCACCGGC-UUGGAc -3' miRNA: 3'- auAGUUAGACGC----GUGGCCGcGGCCUu -5' |
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26671 | 5' | -57.7 | NC_005808.1 | + | 11957 | 0.67 | 0.377826 |
Target: 5'- aAUUggUCUGCGC-CCucauuGCGCCGGc- -3' miRNA: 3'- aUAGuuAGACGCGuGGc----CGCGGCCuu -5' |
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26671 | 5' | -57.7 | NC_005808.1 | + | 7777 | 0.67 | 0.372422 |
Target: 5'- --gCGAUCUGCGCcggcguccacccuucGCCgcccagcaGGCGCUGGAu -3' miRNA: 3'- auaGUUAGACGCG---------------UGG--------CCGCGGCCUu -5' |
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26671 | 5' | -57.7 | NC_005808.1 | + | 28316 | 0.69 | 0.294791 |
Target: 5'- -----cUCgGCGCGCuCGGCGUCGGAc -3' miRNA: 3'- auaguuAGaCGCGUG-GCCGCGGCCUu -5' |
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26671 | 5' | -57.7 | NC_005808.1 | + | 14528 | 0.69 | 0.287317 |
Target: 5'- aAUCAGcca-CGuCGCCGGCGCCGGAc -3' miRNA: 3'- aUAGUUagacGC-GUGGCCGCGGCCUu -5' |
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26671 | 5' | -57.7 | NC_005808.1 | + | 22784 | 0.69 | 0.287317 |
Target: 5'- gGUCAAcgccCUGCGgGCCGGUcagcaugguGCCGGAc -3' miRNA: 3'- aUAGUUa---GACGCgUGGCCG---------CGGCCUu -5' |
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26671 | 5' | -57.7 | NC_005808.1 | + | 6267 | 0.69 | 0.287317 |
Target: 5'- gGUUGAUCUGCGCGCUGGgcagGCCGa-- -3' miRNA: 3'- aUAGUUAGACGCGUGGCCg---CGGCcuu -5' |
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26671 | 5' | -57.7 | NC_005808.1 | + | 23411 | 0.69 | 0.286578 |
Target: 5'- -cUUGAUgaGCGCGCCGGUcagugcgacgccgGCCGGGAu -3' miRNA: 3'- auAGUUAgaCGCGUGGCCG-------------CGGCCUU- -5' |
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26671 | 5' | -57.7 | NC_005808.1 | + | 13802 | 0.71 | 0.203397 |
Target: 5'- gGUCAGUUUGCGCGCCuGUGCCu--- -3' miRNA: 3'- aUAGUUAGACGCGUGGcCGCGGccuu -5' |
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26671 | 5' | -57.7 | NC_005808.1 | + | 13294 | 0.72 | 0.192552 |
Target: 5'- --cCGAUUgcaGCGCGCCGGgGCCGGc- -3' miRNA: 3'- auaGUUAGa--CGCGUGGCCgCGGCCuu -5' |
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26671 | 5' | -57.7 | NC_005808.1 | + | 11263 | 0.78 | 0.066493 |
Target: 5'- ---gGGUUUGCGCugCGGCGCUGGGAc -3' miRNA: 3'- auagUUAGACGCGugGCCGCGGCCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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