miRNA: 3'- -CGCGCGUC-------AGCCGUGA----------ACU--CCGGCCg -5'
miRNA: 3'- cGC-GCGUCAGCCGuGAACUCCGGCcG- -5'
miRNA: 3'- -CGCGCGUCAGCCGUGAACUCCGGCCG- -5'
Results 101 - 103 of 103 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 26672 | 5' | -59.5 | NC_005808.1 | + | 10196 | 0.7 | 0.239287 |
|
Target: 5'- gGCGCGCGGcuuuuuuUCGGUGCUggcuuccugcUGGgcGGCCGGg -3' miRNA: 3'- -CGCGCGUC-------AGCCGUGA----------ACU--CCGGCCg -5' |
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| 26672 | 5' | -59.5 | NC_005808.1 | + | 6670 | 0.71 | 0.216268 |
|
Target: 5'- cCGuCGUAGUUGGC-CUUGAGGUCGcCa -3' miRNA: 3'- cGC-GCGUCAGCCGuGAACUCCGGCcG- -5' |
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| 26672 | 5' | -59.5 | NC_005808.1 | + | 27672 | 1.12 | 0.000178 |
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Target: 5'- gGCGCGCAGUCGGCACUUGAGGCCGGCc -3' miRNA: 3'- -CGCGCGUCAGCCGUGAACUCCGGCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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