Results 1 - 20 of 40 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26674 | 3' | -59.9 | NC_005808.1 | + | 5197 | 0.66 | 0.439297 |
Target: 5'- ---uGCUGGUGCuGGUGGUGCuggCCuGCa -3' miRNA: 3'- ggacCGGCUACG-CCGCCACGua-GG-CG- -5' |
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26674 | 3' | -59.9 | NC_005808.1 | + | 8649 | 0.66 | 0.433606 |
Target: 5'- cCCaGGCCGcgccguacucgcuguGUGCaGCGGcUGCugcgCCGCc -3' miRNA: 3'- -GGaCCGGC---------------UACGcCGCC-ACGua--GGCG- -5' |
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26674 | 3' | -59.9 | NC_005808.1 | + | 4352 | 0.66 | 0.420495 |
Target: 5'- gCUGGCCGuUGCGuugcCGGUGCG--CGUg -3' miRNA: 3'- gGACCGGCuACGCc---GCCACGUagGCG- -5' |
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26674 | 3' | -59.9 | NC_005808.1 | + | 1225 | 0.66 | 0.411279 |
Target: 5'- -aUGGCCGAUGaGGCGGUcgaaaGCGgugUCGa -3' miRNA: 3'- ggACCGGCUACgCCGCCA-----CGUa--GGCg -5' |
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26674 | 3' | -59.9 | NC_005808.1 | + | 18544 | 0.66 | 0.411279 |
Target: 5'- gCCUGGCCGAaGuCGaucacguCGGUGgAggCCGCg -3' miRNA: 3'- -GGACCGGCUaC-GCc------GCCACgUa-GGCG- -5' |
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26674 | 3' | -59.9 | NC_005808.1 | + | 41485 | 0.66 | 0.402188 |
Target: 5'- aCCgGGCCGAUGCG-CGa--CAUgCCGCg -3' miRNA: 3'- -GGaCCGGCUACGCcGCcacGUA-GGCG- -5' |
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26674 | 3' | -59.9 | NC_005808.1 | + | 9772 | 0.66 | 0.402188 |
Target: 5'- ---aGCCGuUGCGGCuGGUGUAgUCCGa -3' miRNA: 3'- ggacCGGCuACGCCG-CCACGU-AGGCg -5' |
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26674 | 3' | -59.9 | NC_005808.1 | + | 35209 | 0.66 | 0.402188 |
Target: 5'- gCUGGUgGAaggucGCcGCGGUGCccgCCGCu -3' miRNA: 3'- gGACCGgCUa----CGcCGCCACGua-GGCG- -5' |
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26674 | 3' | -59.9 | NC_005808.1 | + | 36254 | 0.66 | 0.402188 |
Target: 5'- gCCUucgGGCCGGgcgcuuuggcUGgGGCGGaacGCG-CCGCg -3' miRNA: 3'- -GGA---CCGGCU----------ACgCCGCCa--CGUaGGCG- -5' |
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26674 | 3' | -59.9 | NC_005808.1 | + | 5600 | 0.67 | 0.367131 |
Target: 5'- uUUGGCCcacucgGCGG-GGUGCGuUUCGCg -3' miRNA: 3'- gGACCGGcua---CGCCgCCACGU-AGGCG- -5' |
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26674 | 3' | -59.9 | NC_005808.1 | + | 41417 | 0.67 | 0.364588 |
Target: 5'- aCCcGGCCGAccccgGCGGCGagacuauguggggcGUGaCcgCCGCc -3' miRNA: 3'- -GGaCCGGCUa----CGCCGC--------------CAC-GuaGGCG- -5' |
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26674 | 3' | -59.9 | NC_005808.1 | + | 11517 | 0.67 | 0.358702 |
Target: 5'- gCCUGcGCUGGcgugaggcccUGCGaGCGG-GCAUCCu- -3' miRNA: 3'- -GGAC-CGGCU----------ACGC-CGCCaCGUAGGcg -5' |
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26674 | 3' | -59.9 | NC_005808.1 | + | 13485 | 0.67 | 0.3562 |
Target: 5'- aCCUGGgCGAUGCcgaccgcgccuuugGGCGaGguaagcgguuUGCcGUCCGCg -3' miRNA: 3'- -GGACCgGCUACG--------------CCGC-C----------ACG-UAGGCG- -5' |
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26674 | 3' | -59.9 | NC_005808.1 | + | 25785 | 0.67 | 0.3562 |
Target: 5'- -aUGGCCgcGAUGCGcGCGGcGCGcucgguaucguugaUCUGCa -3' miRNA: 3'- ggACCGG--CUACGC-CGCCaCGU--------------AGGCG- -5' |
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26674 | 3' | -59.9 | NC_005808.1 | + | 15105 | 0.67 | 0.350409 |
Target: 5'- --cGGCCGAggccGcCGGCauccucgcguGGUGCGUgCGCg -3' miRNA: 3'- ggaCCGGCUa---C-GCCG----------CCACGUAgGCG- -5' |
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26674 | 3' | -59.9 | NC_005808.1 | + | 1709 | 0.67 | 0.342253 |
Target: 5'- gCCgugGGCCGGcgUGcCGGCGaGgGCGUCCa- -3' miRNA: 3'- -GGa--CCGGCU--AC-GCCGC-CaCGUAGGcg -5' |
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26674 | 3' | -59.9 | NC_005808.1 | + | 37237 | 0.67 | 0.342253 |
Target: 5'- cCCUgGGCgCGGUGCaGGCGGccacGCAg-CGCg -3' miRNA: 3'- -GGA-CCG-GCUACG-CCGCCa---CGUagGCG- -5' |
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26674 | 3' | -59.9 | NC_005808.1 | + | 23770 | 0.68 | 0.326355 |
Target: 5'- aCCgGGCCGAUGCGccGCGGcuacgaagaUGgAUuCCGUg -3' miRNA: 3'- -GGaCCGGCUACGC--CGCC---------ACgUA-GGCG- -5' |
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26674 | 3' | -59.9 | NC_005808.1 | + | 12709 | 0.68 | 0.325575 |
Target: 5'- gCCUGucauGCCGGggggGgGGUGGUGCuagacccGUUCGCa -3' miRNA: 3'- -GGAC----CGGCUa---CgCCGCCACG-------UAGGCG- -5' |
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26674 | 3' | -59.9 | NC_005808.1 | + | 8625 | 0.68 | 0.318614 |
Target: 5'- uUCUcGGCCGcUGCGGUGGcgagGUcgCCGg -3' miRNA: 3'- -GGA-CCGGCuACGCCGCCa---CGuaGGCg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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