Results 1 - 20 of 28 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26675 | 5' | -56.1 | NC_005808.1 | + | 4815 | 0.66 | 0.63834 |
Target: 5'- gCGGgaUUGcUgGCCGGCGACagGUCgCCg -3' miRNA: 3'- -GCCaaAGC-AgUGGCCGCUGgaCAG-GG- -5' |
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26675 | 5' | -56.1 | NC_005808.1 | + | 23250 | 0.66 | 0.63834 |
Target: 5'- aCGGcaauucCGcCGCCuGGCG-CCUGUCCg -3' miRNA: 3'- -GCCaaa---GCaGUGG-CCGCuGGACAGGg -5' |
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26675 | 5' | -56.1 | NC_005808.1 | + | 25323 | 0.66 | 0.63834 |
Target: 5'- cCGGcgcUCGUCGCCGG-GA-UUGUCCa -3' miRNA: 3'- -GCCaa-AGCAGUGGCCgCUgGACAGGg -5' |
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26675 | 5' | -56.1 | NC_005808.1 | + | 6828 | 0.66 | 0.63834 |
Target: 5'- gGGUUUCGUCGuugcccUCGGCGACg-G-CCUu -3' miRNA: 3'- gCCAAAGCAGU------GGCCGCUGgaCaGGG- -5' |
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26675 | 5' | -56.1 | NC_005808.1 | + | 30374 | 0.66 | 0.616421 |
Target: 5'- -----cUGUUGCCGGUGugCUgcGUCCCg -3' miRNA: 3'- gccaaaGCAGUGGCCGCugGA--CAGGG- -5' |
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26675 | 5' | -56.1 | NC_005808.1 | + | 12896 | 0.66 | 0.616421 |
Target: 5'- uGGUgUCGUCGCCuuuGGCGaugcgcucgGCCgcgCCCa -3' miRNA: 3'- gCCAaAGCAGUGG---CCGC---------UGGacaGGG- -5' |
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26675 | 5' | -56.1 | NC_005808.1 | + | 9063 | 0.67 | 0.583666 |
Target: 5'- cCGGaUUCGcCAgCGGUGGCC-GUgCCg -3' miRNA: 3'- -GCCaAAGCaGUgGCCGCUGGaCAgGG- -5' |
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26675 | 5' | -56.1 | NC_005808.1 | + | 9015 | 0.67 | 0.583666 |
Target: 5'- cCGGaUUCGcCAgCGGUGGCC-GUgCCg -3' miRNA: 3'- -GCCaAAGCaGUgGCCGCUGGaCAgGG- -5' |
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26675 | 5' | -56.1 | NC_005808.1 | + | 8950 | 0.67 | 0.583666 |
Target: 5'- cCGGaUUCGcCAgCGGUGGCC-GUgCCg -3' miRNA: 3'- -GCCaAAGCaGUgGCCGCUGGaCAgGG- -5' |
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26675 | 5' | -56.1 | NC_005808.1 | + | 14415 | 0.67 | 0.583666 |
Target: 5'- uGGg--CGUCGCCaGGCGcGCCUGcggCaCCg -3' miRNA: 3'- gCCaaaGCAGUGG-CCGC-UGGACa--G-GG- -5' |
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26675 | 5' | -56.1 | NC_005808.1 | + | 12023 | 0.67 | 0.583666 |
Target: 5'- gCGGc--CGUgGCCGGCGAgguCUUG-CCCa -3' miRNA: 3'- -GCCaaaGCAgUGGCCGCU---GGACaGGG- -5' |
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26675 | 5' | -56.1 | NC_005808.1 | + | 30988 | 0.67 | 0.572817 |
Target: 5'- aCGGUggaCGaUCugCucacgcGCGACCUGUCCa -3' miRNA: 3'- -GCCAaa-GC-AGugGc-----CGCUGGACAGGg -5' |
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26675 | 5' | -56.1 | NC_005808.1 | + | 34562 | 0.67 | 0.570652 |
Target: 5'- gCGGUUgagcgucgaggcUGUCACCGGCGAagCUGgcgCCa -3' miRNA: 3'- -GCCAAa-----------GCAGUGGCCGCUg-GACa--GGg -5' |
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26675 | 5' | -56.1 | NC_005808.1 | + | 18460 | 0.68 | 0.540588 |
Target: 5'- aCGGUcgCGgCGCCGGCGGCgUcggccgauucGUCCa -3' miRNA: 3'- -GCCAaaGCaGUGGCCGCUGgA----------CAGGg -5' |
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26675 | 5' | -56.1 | NC_005808.1 | + | 31039 | 0.68 | 0.540588 |
Target: 5'- uGGcUUCGaUgACCGGCGGgCaGUCCUa -3' miRNA: 3'- gCCaAAGC-AgUGGCCGCUgGaCAGGG- -5' |
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26675 | 5' | -56.1 | NC_005808.1 | + | 25839 | 0.68 | 0.540588 |
Target: 5'- -----aCGUCGCCGGUGGCUacgCCCa -3' miRNA: 3'- gccaaaGCAGUGGCCGCUGGacaGGG- -5' |
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26675 | 5' | -56.1 | NC_005808.1 | + | 5917 | 0.68 | 0.529976 |
Target: 5'- cCGGcaucuUUUCGcCGCUGGCGGCa--UCCCa -3' miRNA: 3'- -GCC-----AAAGCaGUGGCCGCUGgacAGGG- -5' |
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26675 | 5' | -56.1 | NC_005808.1 | + | 22614 | 0.68 | 0.49863 |
Target: 5'- gCGaGUUcuacgCGgaCAUCGGCGGCCUG-CCCg -3' miRNA: 3'- -GC-CAAa----GCa-GUGGCCGCUGGACaGGG- -5' |
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26675 | 5' | -56.1 | NC_005808.1 | + | 28051 | 0.68 | 0.497599 |
Target: 5'- gGGUUucUCGUCgcuguuguccggcGCCGGCGACgUGgcugauUCCUg -3' miRNA: 3'- gCCAA--AGCAG-------------UGGCCGCUGgAC------AGGG- -5' |
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26675 | 5' | -56.1 | NC_005808.1 | + | 20836 | 0.69 | 0.468138 |
Target: 5'- -----cCGU-GCCgGGCGGCCUGUCCUa -3' miRNA: 3'- gccaaaGCAgUGG-CCGCUGGACAGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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