Results 1 - 18 of 18 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26676 | 3' | -59.7 | NC_005808.1 | + | 37066 | 0.66 | 0.38614 |
Target: 5'- gACC-UgGGCGgaaccuggGCGGCCGgCGuGUCCUAc -3' miRNA: 3'- -UGGuAgUCGCa-------CGCCGGC-GU-CAGGAU- -5' |
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26676 | 3' | -59.7 | NC_005808.1 | + | 6585 | 0.66 | 0.38614 |
Target: 5'- cGCC-UCGGCcagGgGGCUGCGGUCUUu -3' miRNA: 3'- -UGGuAGUCGca-CgCCGGCGUCAGGAu -5' |
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26676 | 3' | -59.7 | NC_005808.1 | + | 27501 | 0.66 | 0.368392 |
Target: 5'- aGCUAUuaCGGCGcGCGGUCGCAGcaaagcagCCUGc -3' miRNA: 3'- -UGGUA--GUCGCaCGCCGGCGUCa-------GGAU- -5' |
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26676 | 3' | -59.7 | NC_005808.1 | + | 27423 | 0.66 | 0.368392 |
Target: 5'- cGCCuggcgCAccGCGcccgGCGGCCGCAGgcCCUGg -3' miRNA: 3'- -UGGua---GU--CGCa---CGCCGGCGUCa-GGAU- -5' |
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26676 | 3' | -59.7 | NC_005808.1 | + | 29048 | 0.66 | 0.368392 |
Target: 5'- cGCCGUgGGCaccgagucagGCGGCCGaCAGUUCg- -3' miRNA: 3'- -UGGUAgUCGca--------CGCCGGC-GUCAGGau -5' |
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26676 | 3' | -59.7 | NC_005808.1 | + | 18470 | 0.66 | 0.351211 |
Target: 5'- cGCCggCGGCGU-CGGCCGauucGUCCa- -3' miRNA: 3'- -UGGuaGUCGCAcGCCGGCgu--CAGGau -5' |
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26676 | 3' | -59.7 | NC_005808.1 | + | 37811 | 0.67 | 0.342837 |
Target: 5'- cGCCAgcCGGCGUGCuGGgCGCAGUgUg- -3' miRNA: 3'- -UGGUa-GUCGCACG-CCgGCGUCAgGau -5' |
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26676 | 3' | -59.7 | NC_005808.1 | + | 14286 | 0.67 | 0.334608 |
Target: 5'- uGCUGUCGGCGaUGCGGCC-CAcGUCg-- -3' miRNA: 3'- -UGGUAGUCGC-ACGCCGGcGU-CAGgau -5' |
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26676 | 3' | -59.7 | NC_005808.1 | + | 39065 | 0.67 | 0.326524 |
Target: 5'- gACUGcgCGGUGUGCGGCUGUAuGUCUa- -3' miRNA: 3'- -UGGUa-GUCGCACGCCGGCGU-CAGGau -5' |
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26676 | 3' | -59.7 | NC_005808.1 | + | 34877 | 0.67 | 0.310793 |
Target: 5'- gGCCGcCgAGCGUGC-GCUGCAGUUCa- -3' miRNA: 3'- -UGGUaG-UCGCACGcCGGCGUCAGGau -5' |
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26676 | 3' | -59.7 | NC_005808.1 | + | 9752 | 0.68 | 0.283946 |
Target: 5'- gACCGUCuGCGgccaggcgaagccguUGCGGCUgguGUAGUCCg- -3' miRNA: 3'- -UGGUAGuCGC---------------ACGCCGG---CGUCAGGau -5' |
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26676 | 3' | -59.7 | NC_005808.1 | + | 21740 | 0.68 | 0.281079 |
Target: 5'- cACCGUUGG-GcGCGGCCGC-GUCCa- -3' miRNA: 3'- -UGGUAGUCgCaCGCCGGCGuCAGGau -5' |
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26676 | 3' | -59.7 | NC_005808.1 | + | 9688 | 0.68 | 0.267088 |
Target: 5'- aGCCGUgCAGgccGUGGCCGCAGUUCUu -3' miRNA: 3'- -UGGUA-GUCgcaCGCCGGCGUCAGGAu -5' |
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26676 | 3' | -59.7 | NC_005808.1 | + | 26173 | 0.69 | 0.247164 |
Target: 5'- cGCCGcCGGCGauuaccccGCGGCCGU-GUCCUAc -3' miRNA: 3'- -UGGUaGUCGCa-------CGCCGGCGuCAGGAU- -5' |
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26676 | 3' | -59.7 | NC_005808.1 | + | 41215 | 0.69 | 0.234576 |
Target: 5'- aACCGUUGuGCGcGUGGCCGCGGcgcCCUAu -3' miRNA: 3'- -UGGUAGU-CGCaCGCCGGCGUCa--GGAU- -5' |
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26676 | 3' | -59.7 | NC_005808.1 | + | 7241 | 0.71 | 0.169977 |
Target: 5'- cACCcUCGGCGaUGuCGGCCGCccaGGUCUUGa -3' miRNA: 3'- -UGGuAGUCGC-AC-GCCGGCG---UCAGGAU- -5' |
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26676 | 3' | -59.7 | NC_005808.1 | + | 26163 | 0.71 | 0.160896 |
Target: 5'- cGCCGUCuGCGaUGCGGUCGCAGauUUCg- -3' miRNA: 3'- -UGGUAGuCGC-ACGCCGGCGUC--AGGau -5' |
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26676 | 3' | -59.7 | NC_005808.1 | + | 26455 | 1.06 | 0.000378 |
Target: 5'- cACCAUCAGCGUGCGGCCGCAGUCCUAc -3' miRNA: 3'- -UGGUAGUCGCACGCCGGCGUCAGGAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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