Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26676 | 5' | -58.7 | NC_005808.1 | + | 8585 | 0.66 | 0.479138 |
Target: 5'- gGCCGcGCGUGCGGCCuGGUgcUGGa-- -3' miRNA: 3'- gUGGC-UGCACGUCGG-CCAgcACCagu -5' |
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26676 | 5' | -58.7 | NC_005808.1 | + | 22974 | 0.66 | 0.463144 |
Target: 5'- -uCCuGCGU-CAGCCGGUCGgauucggcuuucgccUGGUCGg -3' miRNA: 3'- guGGcUGCAcGUCGGCCAGC---------------ACCAGU- -5' |
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26676 | 5' | -58.7 | NC_005808.1 | + | 11159 | 0.66 | 0.459189 |
Target: 5'- gGCCGccugcgcacguuGCGUGCgcuGGUCGGcCGUGGUg- -3' miRNA: 3'- gUGGC------------UGCACG---UCGGCCaGCACCAgu -5' |
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26676 | 5' | -58.7 | NC_005808.1 | + | 27584 | 0.67 | 0.420673 |
Target: 5'- gCACCGGCa-GCAGgCGGcgccagauggCGUGGUCGu -3' miRNA: 3'- -GUGGCUGcaCGUCgGCCa---------GCACCAGU- -5' |
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26676 | 5' | -58.7 | NC_005808.1 | + | 24596 | 0.67 | 0.3931 |
Target: 5'- -uCCGGCGUGUccuuGCCGGg-GUaGGUCAg -3' miRNA: 3'- guGGCUGCACGu---CGGCCagCA-CCAGU- -5' |
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26676 | 5' | -58.7 | NC_005808.1 | + | 7264 | 0.68 | 0.333506 |
Target: 5'- uCGCCGGCcuuGUGCAGCuucuCGG-CGgUGGUCGg -3' miRNA: 3'- -GUGGCUG---CACGUCG----GCCaGC-ACCAGU- -5' |
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26676 | 5' | -58.7 | NC_005808.1 | + | 21747 | 0.69 | 0.31774 |
Target: 5'- gCGCCGuCGUGCAGUCGGUCa------ -3' miRNA: 3'- -GUGGCuGCACGUCGGCCAGcaccagu -5' |
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26676 | 5' | -58.7 | NC_005808.1 | + | 31510 | 0.7 | 0.28151 |
Target: 5'- cCGCCGGCGUGCuGCUGGgccucgcuguguugcCGUGGa-- -3' miRNA: 3'- -GUGGCUGCACGuCGGCCa--------------GCACCagu -5' |
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26676 | 5' | -58.7 | NC_005808.1 | + | 21819 | 0.7 | 0.273841 |
Target: 5'- aCAUgGACGUGCAGCCGcG-CGaUGaGUCGa -3' miRNA: 3'- -GUGgCUGCACGUCGGC-CaGC-AC-CAGU- -5' |
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26676 | 5' | -58.7 | NC_005808.1 | + | 4401 | 0.7 | 0.267015 |
Target: 5'- gCGCCGggccuugaGCGUGCGGCa-GUCGUaGGUCGu -3' miRNA: 3'- -GUGGC--------UGCACGUCGgcCAGCA-CCAGU- -5' |
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26676 | 5' | -58.7 | NC_005808.1 | + | 33284 | 0.71 | 0.234938 |
Target: 5'- gCGCCGACGUGCGccGCCaGGUCGccgaggaaGUCAa -3' miRNA: 3'- -GUGGCUGCACGU--CGG-CCAGCac------CAGU- -5' |
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26676 | 5' | -58.7 | NC_005808.1 | + | 21699 | 0.71 | 0.22304 |
Target: 5'- gCAUCGACacguugccgGCAGUCGGUucCGUGGUCGa -3' miRNA: 3'- -GUGGCUGca-------CGUCGGCCA--GCACCAGU- -5' |
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26676 | 5' | -58.7 | NC_005808.1 | + | 14033 | 0.72 | 0.200782 |
Target: 5'- gGCCGACGUGCgcgccgcgauGGCCGG-CGgccgcaaGGUCGu -3' miRNA: 3'- gUGGCUGCACG----------UCGGCCaGCa------CCAGU- -5' |
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26676 | 5' | -58.7 | NC_005808.1 | + | 6211 | 0.73 | 0.171027 |
Target: 5'- --aCGGCGcGCGGCUccuGGUCGUGGUCu -3' miRNA: 3'- gugGCUGCaCGUCGG---CCAGCACCAGu -5' |
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26676 | 5' | -58.7 | NC_005808.1 | + | 26490 | 1.08 | 0.000415 |
Target: 5'- cCACCGACGUGCAGCCGGUCGUGGUCAa -3' miRNA: 3'- -GUGGCUGCACGUCGGCCAGCACCAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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