Results 101 - 103 of 103 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26677 | 3' | -54.5 | NC_005808.1 | + | 41868 | 0.68 | 0.554146 |
Target: 5'- ---gGUCAUCCGCCAggcgcUCGacuugGCCGUg -3' miRNA: 3'- guugCGGUAGGCGGUau---AGCa----CGGCG- -5' |
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26677 | 3' | -54.5 | NC_005808.1 | + | 41970 | 0.71 | 0.399733 |
Target: 5'- uCGACGCCGgaCGCCGUGUCcacggcGCUGCu -3' miRNA: 3'- -GUUGCGGUagGCGGUAUAGca----CGGCG- -5' |
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26677 | 3' | -54.5 | NC_005808.1 | + | 42391 | 0.72 | 0.329367 |
Target: 5'- -uGCGCCAgUUCGCCGUGUCGcccgGCaGCg -3' miRNA: 3'- guUGCGGU-AGGCGGUAUAGCa---CGgCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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