Results 1 - 18 of 18 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26678 | 3' | -56.7 | NC_005808.1 | + | 31901 | 0.66 | 0.541488 |
Target: 5'- uCCGCcaucgaGGCCGaGUgCCUGCggcguUUCGAGCGc -3' miRNA: 3'- -GGCG------CCGGCaCA-GGAUG-----AAGCUCGUu -5' |
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26678 | 3' | -56.7 | NC_005808.1 | + | 10819 | 0.66 | 0.538245 |
Target: 5'- gCGCGGCUGaaUCCUuccgGCUcggucuugagguggUCGAGCAGg -3' miRNA: 3'- gGCGCCGGCacAGGA----UGA--------------AGCUCGUU- -5' |
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26678 | 3' | -56.7 | NC_005808.1 | + | 21293 | 0.66 | 0.530706 |
Target: 5'- cCCaGCGGUCGgcGUCgaACUugUCGAGCAc -3' miRNA: 3'- -GG-CGCCGGCa-CAGgaUGA--AGCUCGUu -5' |
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26678 | 3' | -56.7 | NC_005808.1 | + | 7555 | 0.67 | 0.509385 |
Target: 5'- cUCGCGGCCGgGUCgUACUuaaUCGgauAGUAGc -3' miRNA: 3'- -GGCGCCGGCaCAGgAUGA---AGC---UCGUU- -5' |
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26678 | 3' | -56.7 | NC_005808.1 | + | 39794 | 0.67 | 0.488433 |
Target: 5'- -aGcCGGUUcUGUCCUGCUUCG-GCGAa -3' miRNA: 3'- ggC-GCCGGcACAGGAUGAAGCuCGUU- -5' |
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26678 | 3' | -56.7 | NC_005808.1 | + | 37544 | 0.67 | 0.488433 |
Target: 5'- gCCGCGGCCuGUGcUCaagcaggACgccaUCGAGCGGg -3' miRNA: 3'- -GGCGCCGG-CAC-AGga-----UGa---AGCUCGUU- -5' |
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26678 | 3' | -56.7 | NC_005808.1 | + | 14767 | 0.67 | 0.467894 |
Target: 5'- gCGCGGCCGgcgCC-ACcgCGGGCAGc -3' miRNA: 3'- gGCGCCGGCacaGGaUGaaGCUCGUU- -5' |
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26678 | 3' | -56.7 | NC_005808.1 | + | 10023 | 0.68 | 0.457792 |
Target: 5'- gCGuCGGCgGUGUCCUGauacgUCgGAGCGg -3' miRNA: 3'- gGC-GCCGgCACAGGAUga---AG-CUCGUu -5' |
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26678 | 3' | -56.7 | NC_005808.1 | + | 10515 | 0.68 | 0.447809 |
Target: 5'- aCGCGGCCGUGUUg-GCggggccgaUGAGCAu -3' miRNA: 3'- gGCGCCGGCACAGgaUGaa------GCUCGUu -5' |
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26678 | 3' | -56.7 | NC_005808.1 | + | 15446 | 0.68 | 0.42821 |
Target: 5'- gUGCGGCgCGUGggggACUUCGAGUGAu -3' miRNA: 3'- gGCGCCG-GCACaggaUGAAGCUCGUU- -5' |
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26678 | 3' | -56.7 | NC_005808.1 | + | 32634 | 0.69 | 0.399788 |
Target: 5'- gCCGCGGUCGcUGUCCUG--UCGAaguucggcgccGCGAa -3' miRNA: 3'- -GGCGCCGGC-ACAGGAUgaAGCU-----------CGUU- -5' |
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26678 | 3' | -56.7 | NC_005808.1 | + | 40560 | 0.69 | 0.390585 |
Target: 5'- uUGCGcGCCGUGUUCUaucgccugcgcgACUUCGccAGCGAg -3' miRNA: 3'- gGCGC-CGGCACAGGA------------UGAAGC--UCGUU- -5' |
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26678 | 3' | -56.7 | NC_005808.1 | + | 37083 | 0.69 | 0.363821 |
Target: 5'- -gGCGGCCGgcgUGUCCUacgACUUUG-GCAGc -3' miRNA: 3'- ggCGCCGGC---ACAGGA---UGAAGCuCGUU- -5' |
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26678 | 3' | -56.7 | NC_005808.1 | + | 14526 | 0.71 | 0.284074 |
Target: 5'- gCCGCGGCCaccUG-CCcGCUguUCGAGCAGa -3' miRNA: 3'- -GGCGCCGGc--ACaGGaUGA--AGCUCGUU- -5' |
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26678 | 3' | -56.7 | NC_005808.1 | + | 26465 | 0.71 | 0.276907 |
Target: 5'- gUGCGGCCGcaGUCCUACgUCGGGg-- -3' miRNA: 3'- gGCGCCGGCa-CAGGAUGaAGCUCguu -5' |
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26678 | 3' | -56.7 | NC_005808.1 | + | 9698 | 0.71 | 0.269885 |
Target: 5'- gCCGUGGCCGcaguucuuguUGUCCUuccagUCGGGCGc -3' miRNA: 3'- -GGCGCCGGC----------ACAGGAuga--AGCUCGUu -5' |
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26678 | 3' | -56.7 | NC_005808.1 | + | 8230 | 0.72 | 0.256273 |
Target: 5'- gCGCGGCCGUGcCCUcg-UCG-GCGAa -3' miRNA: 3'- gGCGCCGGCACaGGAugaAGCuCGUU- -5' |
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26678 | 3' | -56.7 | NC_005808.1 | + | 26189 | 1.07 | 0.000655 |
Target: 5'- cCCGCGGCCGUGUCCUACUUCGAGCAAc -3' miRNA: 3'- -GGCGCCGGCACAGGAUGAAGCUCGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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