Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2668 | 3' | -50 | NC_001491.2 | + | 25666 | 0.66 | 0.996432 |
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Target: 5'- -uUGCACGucaCUGAAgacgcCUUCCAGGAa -3' miRNA: 3'- guAUGUGCcc-GACUUau---GAAGGUCCU- -5' |
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| 2668 | 3' | -50 | NC_001491.2 | + | 11939 | 0.66 | 0.996432 |
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Target: 5'- --gGCACGGcaccucGCUGggUGCgaCgGGGAa -3' miRNA: 3'- guaUGUGCC------CGACuuAUGaaGgUCCU- -5' |
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| 2668 | 3' | -50 | NC_001491.2 | + | 5322 | 0.66 | 0.995089 |
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Target: 5'- --aGC-CGGGCUGggUG---CCGGGGa -3' miRNA: 3'- guaUGuGCCCGACuuAUgaaGGUCCU- -5' |
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| 2668 | 3' | -50 | NC_001491.2 | + | 3303 | 0.66 | 0.995089 |
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Target: 5'- --aACACGGcCUGggUGacggUCCAGGc -3' miRNA: 3'- guaUGUGCCcGACuuAUga--AGGUCCu -5' |
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| 2668 | 3' | -50 | NC_001491.2 | + | 119752 | 0.66 | 0.995013 |
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Target: 5'- aCAUACGCGGauucugaacaugcGCgGGAUGCUggCCAGaGAc -3' miRNA: 3'- -GUAUGUGCC-------------CGaCUUAUGAa-GGUC-CU- -5' |
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| 2668 | 3' | -50 | NC_001491.2 | + | 13372 | 0.67 | 0.993362 |
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Target: 5'- --aGCugGGGUUGGAaACUgaCAGGGg -3' miRNA: 3'- guaUGugCCCGACUUaUGAagGUCCU- -5' |
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| 2668 | 3' | -50 | NC_001491.2 | + | 115339 | 0.68 | 0.981147 |
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Target: 5'- uCAUAUACGGGCgcgGugccAUGCUgcgcUUCAGGGu -3' miRNA: 3'- -GUAUGUGCCCGa--Cu---UAUGA----AGGUCCU- -5' |
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| 2668 | 3' | -50 | NC_001491.2 | + | 37238 | 0.71 | 0.917083 |
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Target: 5'- --gACGCGGGCUGGGgggg-CCAGGc -3' miRNA: 3'- guaUGUGCCCGACUUaugaaGGUCCu -5' |
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| 2668 | 3' | -50 | NC_001491.2 | + | 77201 | 1.1 | 0.00889 |
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Target: 5'- cCAUACACGGGCUGAAUACUUCCAGGAg -3' miRNA: 3'- -GUAUGUGCCCGACUUAUGAAGGUCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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