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miRNA: 3'- -GU-GGGGCGgCCGUu--GCuCCGG-------------CCGGU- -5'
miRNA: 3'- -GUGGGGcGgCCG-UUGCUCCgg-----CCGGU- -5'
Results 41 - 42 of 42 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio#
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P value |
| Predicted miRNA align pattern | |||||||
| 2668 | 5' | -65.1 | NC_001491.2 | + | 4683 | 0.66 | 0.465054 |
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Target: 5'- cCAuCCCCGCgGGCGguuCG-GGCCucuccagcgucuuGGCCAg -3' miRNA: 3'- -GU-GGGGCGgCCGUu--GCuCCGG-------------CCGGU- -5' |
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| 2668 | 5' | -65.1 | NC_001491.2 | + | 2793 | 0.66 | 0.52016 |
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Target: 5'- gUACCCC-CgGGCgGACGAGGagaaggaGGCCGa -3' miRNA: 3'- -GUGGGGcGgCCG-UUGCUCCgg-----CCGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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