Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26685 | 3' | -52.7 | NC_005808.1 | + | 38596 | 0.66 | 0.810089 |
Target: 5'- uGGCCGgCGCGUUUU-GG-GC-GGACa -3' miRNA: 3'- gCUGGCaGUGCAAAAgCCaCGuCCUG- -5' |
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26685 | 3' | -52.7 | NC_005808.1 | + | 40084 | 0.66 | 0.804222 |
Target: 5'- uGACCGgCACGcccagcccgaaCGGguUGCAGGACu -3' miRNA: 3'- gCUGGCaGUGCaaaa-------GCC--ACGUCCUG- -5' |
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26685 | 3' | -52.7 | NC_005808.1 | + | 24332 | 0.66 | 0.780094 |
Target: 5'- aCGGCCGgCAaGUUcUCGGcGCAGGuCg -3' miRNA: 3'- -GCUGGCaGUgCAAaAGCCaCGUCCuG- -5' |
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26685 | 3' | -52.7 | NC_005808.1 | + | 41296 | 0.66 | 0.770795 |
Target: 5'- uGGCCGUCGCccugggcgcggCGGUGU-GGGCg -3' miRNA: 3'- gCUGGCAGUGcaaaa------GCCACGuCCUG- -5' |
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26685 | 3' | -52.7 | NC_005808.1 | + | 34487 | 0.67 | 0.759265 |
Target: 5'- -cGCCGgcCGCG----CGGUGCAGGGCc -3' miRNA: 3'- gcUGGCa-GUGCaaaaGCCACGUCCUG- -5' |
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26685 | 3' | -52.7 | NC_005808.1 | + | 34399 | 0.67 | 0.724832 |
Target: 5'- uGGCCGcCAUGUcgcugacgaaCGG-GCAGGACg -3' miRNA: 3'- gCUGGCaGUGCAaaa-------GCCaCGUCCUG- -5' |
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26685 | 3' | -52.7 | NC_005808.1 | + | 16111 | 0.69 | 0.648836 |
Target: 5'- aCGACCGgcugCACG---UCGGUggccccgacGUAGGACu -3' miRNA: 3'- -GCUGGCa---GUGCaaaAGCCA---------CGUCCUG- -5' |
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26685 | 3' | -52.7 | NC_005808.1 | + | 3728 | 0.69 | 0.637509 |
Target: 5'- cCGuGCCGuUCACGc---CGGUGguGGGCa -3' miRNA: 3'- -GC-UGGC-AGUGCaaaaGCCACguCCUG- -5' |
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26685 | 3' | -52.7 | NC_005808.1 | + | 8584 | 0.69 | 0.626176 |
Target: 5'- aGGCCG-CGCGUgcggccUGGUGCuGGAUa -3' miRNA: 3'- gCUGGCaGUGCAaaa---GCCACGuCCUG- -5' |
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26685 | 3' | -52.7 | NC_005808.1 | + | 16148 | 0.71 | 0.482768 |
Target: 5'- gCGGCCG-CACGcUgaUGGUGguGGGCg -3' miRNA: 3'- -GCUGGCaGUGCaAaaGCCACguCCUG- -5' |
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26685 | 3' | -52.7 | NC_005808.1 | + | 4354 | 0.72 | 0.471281 |
Target: 5'- uGGCCGUUGCGUUgcCGGUGCgcgugaaGGcGACg -3' miRNA: 3'- gCUGGCAGUGCAAaaGCCACG-------UC-CUG- -5' |
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26685 | 3' | -52.7 | NC_005808.1 | + | 24155 | 1.1 | 0.001267 |
Target: 5'- gCGACCGUCACGUUUUCGGUGCAGGACu -3' miRNA: 3'- -GCUGGCAGUGCAAAAGCCACGUCCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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