Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26687 | 3' | -55.8 | NC_005808.1 | + | 26859 | 0.66 | 0.611846 |
Target: 5'- aGGUGcggUAUGUCGagCGCAUgGCCu- -3' miRNA: 3'- aCCGCa--AUGCAGCagGCGUAgCGGuu -5' |
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26687 | 3' | -55.8 | NC_005808.1 | + | 28290 | 0.66 | 0.611846 |
Target: 5'- aGGCGcgcgccagUUcagcaacgACGUgGgCCGCAUCGCCGAc -3' miRNA: 3'- aCCGC--------AA--------UGCAgCaGGCGUAGCGGUU- -5' |
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26687 | 3' | -55.8 | NC_005808.1 | + | 19148 | 0.66 | 0.589425 |
Target: 5'- cGGCGUgUACGcgCGaaccaUCCGCAUCccgGCCGg -3' miRNA: 3'- aCCGCA-AUGCa-GC-----AGGCGUAG---CGGUu -5' |
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26687 | 3' | -55.8 | NC_005808.1 | + | 7544 | 0.66 | 0.589425 |
Target: 5'- cGGCGgcgUGC-UCGaUCUGCggCGCCAc -3' miRNA: 3'- aCCGCa--AUGcAGC-AGGCGuaGCGGUu -5' |
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26687 | 3' | -55.8 | NC_005808.1 | + | 6989 | 0.66 | 0.578267 |
Target: 5'- -cGCGgaaGCG-CGUCCGCGcccgauUCGCCAu -3' miRNA: 3'- acCGCaa-UGCaGCAGGCGU------AGCGGUu -5' |
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26687 | 3' | -55.8 | NC_005808.1 | + | 32298 | 0.67 | 0.545104 |
Target: 5'- aGcGCGUUGCG-CGaCCuCAUCGCCGc -3' miRNA: 3'- aC-CGCAAUGCaGCaGGcGUAGCGGUu -5' |
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26687 | 3' | -55.8 | NC_005808.1 | + | 6537 | 0.68 | 0.491345 |
Target: 5'- cGGgGUcguCGUCGcCCGaAUCGCCAGg -3' miRNA: 3'- aCCgCAau-GCAGCaGGCgUAGCGGUU- -5' |
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26687 | 3' | -55.8 | NC_005808.1 | + | 29682 | 0.68 | 0.491345 |
Target: 5'- -aGCGUUAUGggcgcggcCGagCGCAUCGCCAAa -3' miRNA: 3'- acCGCAAUGCa-------GCagGCGUAGCGGUU- -5' |
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26687 | 3' | -55.8 | NC_005808.1 | + | 14380 | 0.68 | 0.491345 |
Target: 5'- cGcGCGUg--GUCGUCgGCGUgGCCGAc -3' miRNA: 3'- aC-CGCAaugCAGCAGgCGUAgCGGUU- -5' |
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26687 | 3' | -55.8 | NC_005808.1 | + | 37082 | 0.68 | 0.479841 |
Target: 5'- gGGCGgccgGCGU-GUCCuacgacuuuggcaGCAUCGCCGu -3' miRNA: 3'- aCCGCaa--UGCAgCAGG-------------CGUAGCGGUu -5' |
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26687 | 3' | -55.8 | NC_005808.1 | + | 5443 | 0.7 | 0.382985 |
Target: 5'- cGGCGUggGCGagGUCgGUAUCGaCCAu -3' miRNA: 3'- aCCGCAa-UGCagCAGgCGUAGC-GGUu -5' |
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26687 | 3' | -55.8 | NC_005808.1 | + | 5054 | 0.7 | 0.379351 |
Target: 5'- uUGGCGaccgcugcauugAUGUUGUCgGCAUUGCCGAc -3' miRNA: 3'- -ACCGCaa----------UGCAGCAGgCGUAGCGGUU- -5' |
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26687 | 3' | -55.8 | NC_005808.1 | + | 10030 | 0.75 | 0.16137 |
Target: 5'- cGGUGUccugaUACGUCGgagCgGCAUCGCCGg -3' miRNA: 3'- aCCGCA-----AUGCAGCa--GgCGUAGCGGUu -5' |
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26687 | 3' | -55.8 | NC_005808.1 | + | 23629 | 1.06 | 0.001001 |
Target: 5'- cUGGCGUUACGUCGUCCGCAUCGCCAAu -3' miRNA: 3'- -ACCGCAAUGCAGCAGGCGUAGCGGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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