Results 41 - 49 of 49 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26688 | 3' | -57.9 | NC_005808.1 | + | 2569 | 0.66 | 0.472587 |
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Target: 5'- -cGACaCGG-CCCAGcGCGugCGCGCg- -3' miRNA: 3'- acUUG-GUCuGGGUC-CGCugGUGCGac -5' |
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| 26688 | 3' | -57.9 | NC_005808.1 | + | 7168 | 0.66 | 0.482795 |
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Target: 5'- aGGGCCGGGCgcuugucggauUCGGG-GGCgACGCUGg -3' miRNA: 3'- aCUUGGUCUG-----------GGUCCgCUGgUGCGAC- -5' |
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| 26688 | 3' | -57.9 | NC_005808.1 | + | 29010 | 0.66 | 0.493108 |
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Target: 5'- cGAcgUAGGCgaCCAGGCG-CCGCGCUu -3' miRNA: 3'- aCUugGUCUG--GGUCCGCuGGUGCGAc -5' |
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| 26688 | 3' | -57.9 | NC_005808.1 | + | 7691 | 0.66 | 0.493108 |
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Target: 5'- cGGGCUuGACCUcGGCGGCCG-GCUu -3' miRNA: 3'- aCUUGGuCUGGGuCCGCUGGUgCGAc -5' |
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| 26688 | 3' | -57.9 | NC_005808.1 | + | 25134 | 0.66 | 0.493108 |
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Target: 5'- -cGugCAGGCCCuGGCGuggcACCugGCc- -3' miRNA: 3'- acUugGUCUGGGuCCGC----UGGugCGac -5' |
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| 26688 | 3' | -57.9 | NC_005808.1 | + | 32934 | 0.66 | 0.503521 |
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Target: 5'- -cGGCUguucGGCCaGGGCGACCACgGCUGc -3' miRNA: 3'- acUUGGu---CUGGgUCCGCUGGUG-CGAC- -5' |
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| 26688 | 3' | -57.9 | NC_005808.1 | + | 2725 | 0.66 | 0.503521 |
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Target: 5'- -uGGCCGGACgCUugAGcGCGGCCcgGCGCUGc -3' miRNA: 3'- acUUGGUCUG-GG--UC-CGCUGG--UGCGAC- -5' |
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| 26688 | 3' | -57.9 | NC_005808.1 | + | 10234 | 0.66 | 0.503521 |
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Target: 5'- -cGGCCGGGgCCucggcGGCGACCGCaGCg- -3' miRNA: 3'- acUUGGUCUgGGu----CCGCUGGUG-CGac -5' |
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| 26688 | 3' | -57.9 | NC_005808.1 | + | 16969 | 0.66 | 0.503521 |
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Target: 5'- cGAGgUGGACgCGcugcuGGCGACCGCGCc- -3' miRNA: 3'- aCUUgGUCUGgGU-----CCGCUGGUGCGac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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