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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 26688 | 5' | -52.9 | NC_005808.1 | + | 25322 | 0.71 | 0.448883 |
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Target: 5'- aCCGGCGcUCGuCGCCGGGAuuguccaggccggccAGUUCGc -3' miRNA: 3'- aGGUCGU-AGCuGCGGCUCU---------------UCAAGUa -5' |
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| 26688 | 5' | -52.9 | NC_005808.1 | + | 32855 | 0.67 | 0.701051 |
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Target: 5'- cCUGGCcgcagacgGUCGGCGCCGAGAuugUCGc -3' miRNA: 3'- aGGUCG--------UAGCUGCGGCUCUucaAGUa -5' |
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| 26688 | 5' | -52.9 | NC_005808.1 | + | 23341 | 1.07 | 0.00155 |
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Target: 5'- uUCCAGCAUCGACGCCGAGAAGUUCAUg -3' miRNA: 3'- -AGGUCGUAGCUGCGGCUCUUCAAGUA- -5' |
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| 26688 | 5' | -52.9 | NC_005808.1 | + | 32744 | 0.68 | 0.63249 |
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Target: 5'- gCCGGUgccacgcucAUCGACGCCGAaaccGAGGUgcagCGUg -3' miRNA: 3'- aGGUCG---------UAGCUGCGGCU----CUUCAa---GUA- -5' |
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| 26688 | 5' | -52.9 | NC_005808.1 | + | 37033 | 0.66 | 0.73451 |
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Target: 5'- gCCAGCGUUGGCGCCGucGAuac-CGUg -3' miRNA: 3'- aGGUCGUAGCUGCGGCu-CUucaaGUA- -5' |
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| 26688 | 5' | -52.9 | NC_005808.1 | + | 13729 | 0.66 | 0.771192 |
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Target: 5'- gCCAGCAgcgaggacguggcgCGGCGCUGGGAcAGcUUCGg -3' miRNA: 3'- aGGUCGUa-------------GCUGCGGCUCU-UC-AAGUa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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