Results 1 - 18 of 18 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26689 | 5' | -52.5 | NC_005808.1 | + | 26571 | 0.72 | 0.476055 |
Target: 5'- -aCAacUgGcAGGCCAACGGUUucgUCACCGGc -3' miRNA: 3'- caGU--AgC-UCCGGUUGCCAA---AGUGGCU- -5' |
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26689 | 5' | -52.5 | NC_005808.1 | + | 26481 | 0.66 | 0.815507 |
Target: 5'- -aCGUCGGGGCCAcCGacGUgcaGCCGGu -3' miRNA: 3'- caGUAGCUCCGGUuGC--CAaagUGGCU- -5' |
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26689 | 5' | -52.5 | NC_005808.1 | + | 26344 | 0.66 | 0.792746 |
Target: 5'- -gCG-CGAGGCCAACGccauccgccauaucGUgccgcUCACCGAg -3' miRNA: 3'- caGUaGCUCCGGUUGC--------------CAa----AGUGGCU- -5' |
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26689 | 5' | -52.5 | NC_005808.1 | + | 37853 | 0.66 | 0.782544 |
Target: 5'- -aCAUCGcGGCCGuggccgagaacaucGCGGUcaUCAUCGAa -3' miRNA: 3'- caGUAGCuCCGGU--------------UGCCAa-AGUGGCU- -5' |
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26689 | 5' | -52.5 | NC_005808.1 | + | 23417 | 0.67 | 0.732558 |
Target: 5'- aUCAUCGAcGCCGGCGGcacgggCAgCGAc -3' miRNA: 3'- cAGUAGCUcCGGUUGCCaaa---GUgGCU- -5' |
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26689 | 5' | -52.5 | NC_005808.1 | + | 39144 | 0.67 | 0.732558 |
Target: 5'- -aCAUCGGGGCgcGCGGcgUCugUGAc -3' miRNA: 3'- caGUAGCUCCGguUGCCaaAGugGCU- -5' |
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26689 | 5' | -52.5 | NC_005808.1 | + | 4823 | 0.68 | 0.710486 |
Target: 5'- --gGUCGGGGCCGGCGccgUCGUCGAa -3' miRNA: 3'- cagUAGCUCCGGUUGCcaaAGUGGCU- -5' |
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26689 | 5' | -52.5 | NC_005808.1 | + | 30040 | 0.68 | 0.665449 |
Target: 5'- -gCAgcgCGAGGCCGGCaagaaaUUCACCGAc -3' miRNA: 3'- caGUa--GCUCCGGUUGcca---AAGUGGCU- -5' |
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26689 | 5' | -52.5 | NC_005808.1 | + | 19375 | 0.69 | 0.597108 |
Target: 5'- gGUCGguggcCGAGGCCGA-GGccgaaUUCACCGAc -3' miRNA: 3'- -CAGUa----GCUCCGGUUgCCa----AAGUGGCU- -5' |
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26689 | 5' | -52.5 | NC_005808.1 | + | 18159 | 0.72 | 0.465612 |
Target: 5'- uGUCGcCGAGGCCGcgcGCGG---CACCGAc -3' miRNA: 3'- -CAGUaGCUCCGGU---UGCCaaaGUGGCU- -5' |
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26689 | 5' | -52.5 | NC_005808.1 | + | 38286 | 0.72 | 0.451196 |
Target: 5'- aUCGUgGAGGUCAACGGcaagcuggugcagUUCACCGc -3' miRNA: 3'- cAGUAgCUCCGGUUGCCa------------AAGUGGCu -5' |
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26689 | 5' | -52.5 | NC_005808.1 | + | 23068 | 1.1 | 0.001269 |
Target: 5'- cGUCAUCGAGGCCAACGGUUUCACCGAg -3' miRNA: 3'- -CAGUAGCUCCGGUUGCCAAAGUGGCU- -5' |
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26689 | 5' | -52.5 | NC_005808.1 | + | 17454 | 0.67 | 0.72157 |
Target: 5'- -gCAUCGAGGCCA--GGUgccaCGCCa- -3' miRNA: 3'- caGUAGCUCCGGUugCCAaa--GUGGcu -5' |
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26689 | 5' | -52.5 | NC_005808.1 | + | 11570 | 0.66 | 0.819358 |
Target: 5'- gGUCAUCGAagccaugccgcccagGGCCGcCGGcuggcCGCCGGg -3' miRNA: 3'- -CAGUAGCU---------------CCGGUuGCCaaa--GUGGCU- -5' |
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26689 | 5' | -52.5 | NC_005808.1 | + | 18462 | 0.66 | 0.815507 |
Target: 5'- gGUCG-CGGcGCCGGCGGcgUCgGCCGAu -3' miRNA: 3'- -CAGUaGCUcCGGUUGCCaaAG-UGGCU- -5' |
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26689 | 5' | -52.5 | NC_005808.1 | + | 38000 | 0.66 | 0.804749 |
Target: 5'- aUCGcCGAGGCCGAgcaguuccucgccUGGcg-CACCGAg -3' miRNA: 3'- cAGUaGCUCCGGUU-------------GCCaaaGUGGCU- -5' |
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26689 | 5' | -52.5 | NC_005808.1 | + | 4175 | 0.66 | 0.775303 |
Target: 5'- cGUgGUCGAGguguaGCCAGUGGg--CGCCGAa -3' miRNA: 3'- -CAgUAGCUC-----CGGUUGCCaaaGUGGCU- -5' |
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26689 | 5' | -52.5 | NC_005808.1 | + | 19758 | 0.67 | 0.732558 |
Target: 5'- cGUUGUUGccGGCCAcgagGCGGUcUUCACUGAu -3' miRNA: 3'- -CAGUAGCu-CCGGU----UGCCA-AAGUGGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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