Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26690 | 5' | -60.6 | NC_005808.1 | + | 25756 | 0.66 | 0.428894 |
Target: 5'- gCUGGGcGcgCUGCUGGGCggCGGCaacgaUGGc -3' miRNA: 3'- -GGCCCaCaaGAUGGCCCG--GCCGg----ACC- -5' |
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26690 | 5' | -60.6 | NC_005808.1 | + | 1750 | 0.66 | 0.401668 |
Target: 5'- aCCGGGcGgUCaugGCCGGcCUGcGCCUGGc -3' miRNA: 3'- -GGCCCaCaAGa--UGGCCcGGC-CGGACC- -5' |
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26690 | 5' | -60.6 | NC_005808.1 | + | 367 | 0.66 | 0.392839 |
Target: 5'- uCCGGGUGUgcuuggcCUGCCGauugaGCgCGGCCa-- -3' miRNA: 3'- -GGCCCACAa------GAUGGCc----CG-GCCGGacc -5' |
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26690 | 5' | -60.6 | NC_005808.1 | + | 37909 | 0.66 | 0.384138 |
Target: 5'- gCGucGGUGccuUUCUACCGGGgCGGCUa-- -3' miRNA: 3'- gGC--CCAC---AAGAUGGCCCgGCCGGacc -5' |
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26690 | 5' | -60.6 | NC_005808.1 | + | 27877 | 0.66 | 0.384138 |
Target: 5'- cCUGGGg---CUACCGGGCgcagGGCaUGGa -3' miRNA: 3'- -GGCCCacaaGAUGGCCCGg---CCGgACC- -5' |
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26690 | 5' | -60.6 | NC_005808.1 | + | 36196 | 0.67 | 0.334678 |
Target: 5'- aUGGGccgcgaCUacACCGGGCCGGCC-GGc -3' miRNA: 3'- gGCCCacaa--GA--UGGCCCGGCCGGaCC- -5' |
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26690 | 5' | -60.6 | NC_005808.1 | + | 14892 | 0.68 | 0.290041 |
Target: 5'- aCCGGcGgcgagccgCUGCCGGcGCgCGGCCUGu -3' miRNA: 3'- -GGCC-Cacaa----GAUGGCC-CG-GCCGGACc -5' |
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26690 | 5' | -60.6 | NC_005808.1 | + | 17242 | 0.69 | 0.276235 |
Target: 5'- gCCcGGUGgccggCUAUgCGaacuGGCCGGCCUGGa -3' miRNA: 3'- -GGcCCACaa---GAUG-GC----CCGGCCGGACC- -5' |
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26690 | 5' | -60.6 | NC_005808.1 | + | 6215 | 0.69 | 0.256519 |
Target: 5'- gCCGGGUucugcGUUUUGCCuucGGCCagGGCCUGc -3' miRNA: 3'- -GGCCCA-----CAAGAUGGc--CCGG--CCGGACc -5' |
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26690 | 5' | -60.6 | NC_005808.1 | + | 17090 | 0.72 | 0.179375 |
Target: 5'- gCCGGcGcgGUUCUcgGCCGGGCCuuggGGCUUGa -3' miRNA: 3'- -GGCC-Ca-CAAGA--UGGCCCGG----CCGGACc -5' |
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26690 | 5' | -60.6 | NC_005808.1 | + | 12477 | 0.73 | 0.133941 |
Target: 5'- gCGGcGUGUUCUGgCGcGGCCucaucGCCUGGg -3' miRNA: 3'- gGCC-CACAAGAUgGC-CCGGc----CGGACC- -5' |
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26690 | 5' | -60.6 | NC_005808.1 | + | 22803 | 1.1 | 0.000227 |
Target: 5'- uCCGGGUGUUCUACCGGGCCGGCCUGGc -3' miRNA: 3'- -GGCCCACAAGAUGGCCCGGCCGGACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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