Results 1 - 10 of 10 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26691 | 3' | -60.3 | NC_005808.1 | + | 37076 | 0.66 | 0.390408 |
Target: 5'- gAACCUGGGcgGCCggcgUGUCCuACGacuuuggcagcaucGCCGu -3' miRNA: 3'- -UUGGACCCaaCGGa---GCAGG-UGC--------------CGGC- -5' |
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26691 | 3' | -60.3 | NC_005808.1 | + | 992 | 0.66 | 0.365014 |
Target: 5'- cGCCUGGcGgUGCCgugguucaccgcagCGUcgaacaccuggaaggCCACGGCCGg -3' miRNA: 3'- uUGGACC-CaACGGa-------------GCA---------------GGUGCCGGC- -5' |
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26691 | 3' | -60.3 | NC_005808.1 | + | 41288 | 0.67 | 0.302542 |
Target: 5'- cGGCCUGGug-GCCgUCGcCCugGGCgCGg -3' miRNA: 3'- -UUGGACCcaaCGG-AGCaGGugCCG-GC- -5' |
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26691 | 3' | -60.3 | NC_005808.1 | + | 30567 | 0.69 | 0.241084 |
Target: 5'- cACgCUGGGcaaGaCCUCGccggCCACGGCCGc -3' miRNA: 3'- uUG-GACCCaa-C-GGAGCa---GGUGCCGGC- -5' |
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26691 | 3' | -60.3 | NC_005808.1 | + | 10787 | 0.69 | 0.239226 |
Target: 5'- cGGCCUGGGaUUgcauguacugcugcGCCUCGg-CGCGGCUGa -3' miRNA: 3'- -UUGGACCC-AA--------------CGGAGCagGUGCCGGC- -5' |
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26691 | 3' | -60.3 | NC_005808.1 | + | 38546 | 0.71 | 0.171027 |
Target: 5'- -cCCUGGGccgcuggccUGCCgcUCG-CCGCGGCCGg -3' miRNA: 3'- uuGGACCCa--------ACGG--AGCaGGUGCCGGC- -5' |
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26691 | 3' | -60.3 | NC_005808.1 | + | 73 | 0.71 | 0.157677 |
Target: 5'- cGCCUaGGGUUGCCUCGaa-GCGaGCCa -3' miRNA: 3'- uUGGA-CCCAACGGAGCaggUGC-CGGc -5' |
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26691 | 3' | -60.3 | NC_005808.1 | + | 8413 | 0.73 | 0.116448 |
Target: 5'- cGGCCaGGGccgUGCgCUUGUCUACGGCCu -3' miRNA: 3'- -UUGGaCCCa--ACG-GAGCAGGUGCCGGc -5' |
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26691 | 3' | -60.3 | NC_005808.1 | + | 20236 | 0.74 | 0.101235 |
Target: 5'- uGGCCuUGGG-UGCCUUGcUCCugGGUCGg -3' miRNA: 3'- -UUGG-ACCCaACGGAGC-AGGugCCGGC- -5' |
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26691 | 3' | -60.3 | NC_005808.1 | + | 22647 | 1.06 | 0.000337 |
Target: 5'- aAACCUGGGUUGCCUCGUCCACGGCCGa -3' miRNA: 3'- -UUGGACCCAACGGAGCAGGUGCCGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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