Results 1 - 17 of 17 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26697 | 3' | -54.5 | NC_005808.1 | + | 32933 | 0.66 | 0.705053 |
Target: 5'- gCGGcUGUUCgGCCAGGGcgACCAcGGcuGCGUCg -3' miRNA: 3'- -GCC-ACAGG-CGGUUCU--UGGU-CC--UGUAG- -5' |
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26697 | 3' | -54.5 | NC_005808.1 | + | 15337 | 0.66 | 0.693972 |
Target: 5'- gCGGUucauugccUCgGCCAAGAGCCuGGGGCGc- -3' miRNA: 3'- -GCCAc-------AGgCGGUUCUUGG-UCCUGUag -5' |
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26697 | 3' | -54.5 | NC_005808.1 | + | 28600 | 0.66 | 0.682828 |
Target: 5'- uGGUGUCgGUCAGuuccuuGAACCGGGcGCGg- -3' miRNA: 3'- gCCACAGgCGGUU------CUUGGUCC-UGUag -5' |
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26697 | 3' | -54.5 | NC_005808.1 | + | 2800 | 0.66 | 0.671632 |
Target: 5'- -cGUGUUCGCC--GAAgCAGGACAg- -3' miRNA: 3'- gcCACAGGCGGuuCUUgGUCCUGUag -5' |
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26697 | 3' | -54.5 | NC_005808.1 | + | 14360 | 0.66 | 0.660398 |
Target: 5'- gCGGUGUCCu---GGAagGCgGGGGCAUCc -3' miRNA: 3'- -GCCACAGGcgguUCU--UGgUCCUGUAG- -5' |
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26697 | 3' | -54.5 | NC_005808.1 | + | 34078 | 0.67 | 0.626575 |
Target: 5'- uGGgcaaGUUCGCCGGcGACaucaAGGGCAUCg -3' miRNA: 3'- gCCa---CAGGCGGUUcUUGg---UCCUGUAG- -5' |
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26697 | 3' | -54.5 | NC_005808.1 | + | 35330 | 0.67 | 0.604033 |
Target: 5'- -----aCCGCCGAGAagcugcacaagGCCGGcGACAUCg -3' miRNA: 3'- gccacaGGCGGUUCU-----------UGGUC-CUGUAG- -5' |
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26697 | 3' | -54.5 | NC_005808.1 | + | 5600 | 0.67 | 0.592795 |
Target: 5'- uCGaUGUCCGCCucguuGGuAGCCAcGGCGUCg -3' miRNA: 3'- -GCcACAGGCGGu----UC-UUGGUcCUGUAG- -5' |
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26697 | 3' | -54.5 | NC_005808.1 | + | 3426 | 0.68 | 0.581593 |
Target: 5'- uCGGcc-CCGCUAcucaAGAAUCAGGugGUCa -3' miRNA: 3'- -GCCacaGGCGGU----UCUUGGUCCugUAG- -5' |
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26697 | 3' | -54.5 | NC_005808.1 | + | 27791 | 0.68 | 0.548284 |
Target: 5'- aGGcagcgCCGCCGAGGugCAGGuuuccagcgACAUCg -3' miRNA: 3'- gCCaca--GGCGGUUCUugGUCC---------UGUAG- -5' |
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26697 | 3' | -54.5 | NC_005808.1 | + | 8717 | 0.7 | 0.46301 |
Target: 5'- uCGGaUGUCgCGCaccuuGAGCCGGGACAc- -3' miRNA: 3'- -GCC-ACAG-GCGguu--CUUGGUCCUGUag -5' |
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26697 | 3' | -54.5 | NC_005808.1 | + | 41167 | 0.7 | 0.46301 |
Target: 5'- aGGUGUuuGCCAucuuGGGCguGGAUGUg -3' miRNA: 3'- gCCACAggCGGUu---CUUGguCCUGUAg -5' |
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26697 | 3' | -54.5 | NC_005808.1 | + | 36245 | 0.7 | 0.452829 |
Target: 5'- uGGgccgGUUCGCCAcccAGAcgcACCAGGGCGa- -3' miRNA: 3'- gCCa---CAGGCGGU---UCU---UGGUCCUGUag -5' |
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26697 | 3' | -54.5 | NC_005808.1 | + | 33875 | 0.7 | 0.452829 |
Target: 5'- uGGUGUCCcgGCuCAAGGugCGcGACAUCc -3' miRNA: 3'- gCCACAGG--CG-GUUCUugGUcCUGUAG- -5' |
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26697 | 3' | -54.5 | NC_005808.1 | + | 23393 | 0.7 | 0.442771 |
Target: 5'- --cUGUCCGCgGAGAacgGCCAGaACAUCa -3' miRNA: 3'- gccACAGGCGgUUCU---UGGUCcUGUAG- -5' |
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26697 | 3' | -54.5 | NC_005808.1 | + | 18072 | 0.71 | 0.385213 |
Target: 5'- gGGUGcgugcugggCCGCCGAagccggcgcGAACCAGGACGccuUCg -3' miRNA: 3'- gCCACa--------GGCGGUU---------CUUGGUCCUGU---AG- -5' |
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26697 | 3' | -54.5 | NC_005808.1 | + | 20805 | 1.11 | 0.000645 |
Target: 5'- cCGGUGUCCGCCAAGAACCAGGACAUCa -3' miRNA: 3'- -GCCACAGGCGGUUCUUGGUCCUGUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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