Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26697 | 5' | -59.7 | NC_005808.1 | + | 9736 | 0.66 | 0.410158 |
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Target: 5'- gCGGGCGaaCUGcUCgUG-GUGGGCGCGc -3' miRNA: 3'- -GCCCGCcgGAC-AGgAUaCACCUGCGC- -5' |
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| 26697 | 5' | -59.7 | NC_005808.1 | + | 41198 | 0.67 | 0.371909 |
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Target: 5'- gGGGCGGCCaugagugaaaccguUGUgCgc-GUGGcCGCGg -3' miRNA: 3'- gCCCGCCGG--------------ACAgGauaCACCuGCGC- -5' |
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| 26697 | 5' | -59.7 | NC_005808.1 | + | 35357 | 0.68 | 0.325077 |
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Target: 5'- uGGGCGGCCgacaucgCCgaggGUGaGCGCGg -3' miRNA: 3'- gCCCGCCGGaca----GGaua-CACcUGCGC- -5' |
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| 26697 | 5' | -59.7 | NC_005808.1 | + | 40570 | 0.69 | 0.294916 |
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Target: 5'- cCGaGGCGGCCgGUaugCCgGUGcUGGugGCGu -3' miRNA: 3'- -GC-CCGCCGGaCA---GGaUAC-ACCugCGC- -5' |
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| 26697 | 5' | -59.7 | NC_005808.1 | + | 7597 | 0.69 | 0.284887 |
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Target: 5'- aGGGUcagGGUCUGUCCgucugccguggugAUGgucaggGGGCGCGg -3' miRNA: 3'- gCCCG---CCGGACAGGa------------UACa-----CCUGCGC- -5' |
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| 26697 | 5' | -59.7 | NC_005808.1 | + | 20841 | 1.09 | 0.000271 |
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Target: 5'- cCGGGCGGCCUGUCCUAUGUGGACGCGg -3' miRNA: 3'- -GCCCGCCGGACAGGAUACACCUGCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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