Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26698 | 5' | -57.3 | NC_005808.1 | + | 20645 | 1.09 | 0.000436 |
Target: 5'- gUCGUUCCGCGCACUGUGCCCGCGUUGg -3' miRNA: 3'- -AGCAAGGCGCGUGACACGGGCGCAAC- -5' |
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26698 | 5' | -57.3 | NC_005808.1 | + | 33742 | 0.74 | 0.171483 |
Target: 5'- uUCGUggcgaCCGaggccaaCGCGCUG-GCCCGCGUUGc -3' miRNA: 3'- -AGCAa----GGC-------GCGUGACaCGGGCGCAAC- -5' |
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26698 | 5' | -57.3 | NC_005808.1 | + | 28644 | 0.72 | 0.217919 |
Target: 5'- -aGUUCCGCGCGCagugccagaucgGUGCCgGCGg-- -3' miRNA: 3'- agCAAGGCGCGUGa-----------CACGGgCGCaac -5' |
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26698 | 5' | -57.3 | NC_005808.1 | + | 27817 | 0.71 | 0.291172 |
Target: 5'- aCGUcCuCGCGCGCgcUGCCCGCGgUGg -3' miRNA: 3'- aGCAaG-GCGCGUGacACGGGCGCaAC- -5' |
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26698 | 5' | -57.3 | NC_005808.1 | + | 15024 | 0.7 | 0.306122 |
Target: 5'- aUGgugUCCGCGUugguguugGCgaUGCCCGCGUUGa -3' miRNA: 3'- aGCa--AGGCGCG--------UGacACGGGCGCAAC- -5' |
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26698 | 5' | -57.3 | NC_005808.1 | + | 37922 | 0.7 | 0.306122 |
Target: 5'- cCGgcuaUCCgGCGCGCUgGUGCCCGCc--- -3' miRNA: 3'- aGCa---AGG-CGCGUGA-CACGGGCGcaac -5' |
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26698 | 5' | -57.3 | NC_005808.1 | + | 11549 | 0.7 | 0.329636 |
Target: 5'- uUCGggccUUCGCGCACguugGCCCGCGc-- -3' miRNA: 3'- -AGCa---AGGCGCGUGaca-CGGGCGCaac -5' |
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26698 | 5' | -57.3 | NC_005808.1 | + | 35970 | 0.68 | 0.436493 |
Target: 5'- aCGUgUCCGCGCGCaGcGCCaCGCGc-- -3' miRNA: 3'- aGCA-AGGCGCGUGaCaCGG-GCGCaac -5' |
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26698 | 5' | -57.3 | NC_005808.1 | + | 30987 | 0.68 | 0.426843 |
Target: 5'- aUGcUUCCGCugGCACUGcUGCCCgGCGg-- -3' miRNA: 3'- aGC-AAGGCG--CGUGAC-ACGGG-CGCaac -5' |
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26698 | 5' | -57.3 | NC_005808.1 | + | 31187 | 0.67 | 0.466173 |
Target: 5'- aCGggCCGCGCACguucUGCCUGaCGg-- -3' miRNA: 3'- aGCaaGGCGCGUGac--ACGGGC-GCaac -5' |
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26698 | 5' | -57.3 | NC_005808.1 | + | 22536 | 0.67 | 0.496865 |
Target: 5'- aCGcgCagCGCGCGC-GUGCCgGUGUUGu -3' miRNA: 3'- aGCaaG--GCGCGUGaCACGGgCGCAAC- -5' |
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26698 | 5' | -57.3 | NC_005808.1 | + | 23893 | 0.67 | 0.444302 |
Target: 5'- cCGUaCCGaUGCGCUGcugcugaccgagGCCCGCGUg- -3' miRNA: 3'- aGCAaGGC-GCGUGACa-----------CGGGCGCAac -5' |
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26698 | 5' | -57.3 | NC_005808.1 | + | 39380 | 0.66 | 0.507298 |
Target: 5'- aCGgUCCGCGacaauauGCUGcguguugaaaaUGCCCGCGUg- -3' miRNA: 3'- aGCaAGGCGCg------UGAC-----------ACGGGCGCAac -5' |
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26698 | 5' | -57.3 | NC_005808.1 | + | 11171 | 0.66 | 0.528433 |
Target: 5'- aCGUUgCGUGCGCUGgucGgCCGUGgUGa -3' miRNA: 3'- aGCAAgGCGCGUGACa--CgGGCGCaAC- -5' |
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26698 | 5' | -57.3 | NC_005808.1 | + | 21379 | 0.66 | 0.539123 |
Target: 5'- uUCGUcgCaaugGCGCGCUGgGCCUGCGc-- -3' miRNA: 3'- -AGCAa-Gg---CGCGUGACaCGGGCGCaac -5' |
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26698 | 5' | -57.3 | NC_005808.1 | + | 28222 | 0.66 | 0.539123 |
Target: 5'- aCGUUCCGCgGCGCgGaUGCCCcCGc-- -3' miRNA: 3'- aGCAAGGCG-CGUGaC-ACGGGcGCaac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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