Results 1 - 20 of 20 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26699 | 3' | -53.4 | NC_005808.1 | + | 20508 | 1.06 | 0.00175 |
Target: 5'- gUUACGGUCAUUCACGCCAUCGAGCCGc -3' miRNA: 3'- -AAUGCCAGUAAGUGCGGUAGCUCGGC- -5' |
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26699 | 3' | -53.4 | NC_005808.1 | + | 1754 | 0.75 | 0.252052 |
Target: 5'- -gGCGGUCAUggccggccugcgcCugGCgCGUCGGGCCGa -3' miRNA: 3'- aaUGCCAGUAa------------GugCG-GUAGCUCGGC- -5' |
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26699 | 3' | -53.4 | NC_005808.1 | + | 22049 | 0.75 | 0.254762 |
Target: 5'- gUUGCGGUCG-UCGCGCUugcUCGGGUCGc -3' miRNA: 3'- -AAUGCCAGUaAGUGCGGu--AGCUCGGC- -5' |
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26699 | 3' | -53.4 | NC_005808.1 | + | 20345 | 0.7 | 0.482758 |
Target: 5'- -gACGGcCGgcgaaUACGCCAUCGcGGCCGa -3' miRNA: 3'- aaUGCCaGUaa---GUGCGGUAGC-UCGGC- -5' |
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26699 | 3' | -53.4 | NC_005808.1 | + | 8714 | 0.69 | 0.536973 |
Target: 5'- ---gGGUCGgaugUCGCGCaCcUUGAGCCGg -3' miRNA: 3'- aaugCCAGUa---AGUGCG-GuAGCUCGGC- -5' |
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26699 | 3' | -53.4 | NC_005808.1 | + | 15592 | 0.69 | 0.548082 |
Target: 5'- cUGCGGaugCAc-CGCGCCAUCG-GCCa -3' miRNA: 3'- aAUGCCa--GUaaGUGCGGUAGCuCGGc -5' |
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26699 | 3' | -53.4 | NC_005808.1 | + | 18514 | 0.69 | 0.569377 |
Target: 5'- cUGCGGUCAUcCAUGCCggUGuugggauuggccuGGCCGa -3' miRNA: 3'- aAUGCCAGUAaGUGCGGuaGC-------------UCGGC- -5' |
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26699 | 3' | -53.4 | NC_005808.1 | + | 31394 | 0.68 | 0.581801 |
Target: 5'- -gGCGGUCAUgCGCGgCAUUGA-CCGc -3' miRNA: 3'- aaUGCCAGUAaGUGCgGUAGCUcGGC- -5' |
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26699 | 3' | -53.4 | NC_005808.1 | + | 37029 | 0.68 | 0.593143 |
Target: 5'- -gGCGGccagCGUUgGCGCCGUCGAuaCCGu -3' miRNA: 3'- aaUGCCa---GUAAgUGCGGUAGCUc-GGC- -5' |
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26699 | 3' | -53.4 | NC_005808.1 | + | 7375 | 0.68 | 0.615923 |
Target: 5'- ---aGGUCGUgaaUCACGUCGUUGAcGCCc -3' miRNA: 3'- aaugCCAGUA---AGUGCGGUAGCU-CGGc -5' |
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26699 | 3' | -53.4 | NC_005808.1 | + | 34134 | 0.67 | 0.638764 |
Target: 5'- cUGCGG-CAUcgCAgGCCAccUCGAcGCCGa -3' miRNA: 3'- aAUGCCaGUAa-GUgCGGU--AGCU-CGGC- -5' |
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26699 | 3' | -53.4 | NC_005808.1 | + | 17677 | 0.67 | 0.65018 |
Target: 5'- -gGCGGgcugggCGUaggcgaaGCGCCAcUCGGGCCGg -3' miRNA: 3'- aaUGCCa-----GUAag-----UGCGGU-AGCUCGGC- -5' |
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26699 | 3' | -53.4 | NC_005808.1 | + | 3055 | 0.67 | 0.695548 |
Target: 5'- --cUGGUCAgcagCACGUCAagcgccCGAGCCGc -3' miRNA: 3'- aauGCCAGUaa--GUGCGGUa-----GCUCGGC- -5' |
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26699 | 3' | -53.4 | NC_005808.1 | + | 22782 | 0.66 | 0.706756 |
Target: 5'- --uUGGUCA---ACGCCcugCGGGCCGg -3' miRNA: 3'- aauGCCAGUaagUGCGGua-GCUCGGC- -5' |
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26699 | 3' | -53.4 | NC_005808.1 | + | 38410 | 0.66 | 0.717885 |
Target: 5'- -cGCGGcCAacaugCcCGCCAUCGAGgCGg -3' miRNA: 3'- aaUGCCaGUaa---GuGCGGUAGCUCgGC- -5' |
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26699 | 3' | -53.4 | NC_005808.1 | + | 32379 | 0.66 | 0.717885 |
Target: 5'- ---gGGUCAaUUugGaauccgccgaCCAUCGGGCCGg -3' miRNA: 3'- aaugCCAGUaAGugC----------GGUAGCUCGGC- -5' |
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26699 | 3' | -53.4 | NC_005808.1 | + | 5549 | 0.66 | 0.739856 |
Target: 5'- --cUGGUCAaUCACGCgCAuggcuucgaugUCGAGgCCGg -3' miRNA: 3'- aauGCCAGUaAGUGCG-GU-----------AGCUC-GGC- -5' |
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26699 | 3' | -53.4 | NC_005808.1 | + | 20642 | 0.66 | 0.739856 |
Target: 5'- --uCGGUCGUUcCGCGCacugugcccgCGUUGGGCCu -3' miRNA: 3'- aauGCCAGUAA-GUGCG----------GUAGCUCGGc -5' |
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26699 | 3' | -53.4 | NC_005808.1 | + | 40787 | 0.66 | 0.739856 |
Target: 5'- --gUGGUCGggUCAUGCgCAaUGGGCCGa -3' miRNA: 3'- aauGCCAGUa-AGUGCG-GUaGCUCGGC- -5' |
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26699 | 3' | -53.4 | NC_005808.1 | + | 4376 | 0.66 | 0.750672 |
Target: 5'- -gGCGGaugcgCAgccagGCGCCGUCGcGCCGg -3' miRNA: 3'- aaUGCCa----GUaag--UGCGGUAGCuCGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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