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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 26703 | 3' | -48.2 | NC_005808.1 | + | 34552 | 0.72 | 0.656954 |
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Target: 5'- --cGCGCGuCAUGGAcggCGGCAAGgacggcgGCCc -3' miRNA: 3'- uuaUGCGC-GUACUUa--GCCGUUUa------CGG- -5' |
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| 26703 | 3' | -48.2 | NC_005808.1 | + | 12398 | 0.74 | 0.57355 |
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Target: 5'- -cUGCGCGCuUGuaugCGGCcuuGAUGCCg -3' miRNA: 3'- uuAUGCGCGuACuua-GCCGu--UUACGG- -5' |
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| 26703 | 3' | -48.2 | NC_005808.1 | + | 4683 | 0.74 | 0.561772 |
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Target: 5'- --aGCGCGCcgGAuagcgCGGCGAGaaugGCCg -3' miRNA: 3'- uuaUGCGCGuaCUua---GCCGUUUa---CGG- -5' |
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| 26703 | 3' | -48.2 | NC_005808.1 | + | 4371 | 0.74 | 0.561772 |
|
Target: 5'- ---gUGCGCGUGAa--GGCGAcgGCCg -3' miRNA: 3'- uuauGCGCGUACUuagCCGUUuaCGG- -5' |
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| 26703 | 3' | -48.2 | NC_005808.1 | + | 12368 | 0.75 | 0.481697 |
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Target: 5'- gGAUuuGCGCGUcGGUCGGCGagucGAUGCCu -3' miRNA: 3'- -UUAugCGCGUAcUUAGCCGU----UUACGG- -5' |
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| 26703 | 3' | -48.2 | NC_005808.1 | + | 14379 | 0.78 | 0.359188 |
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Target: 5'- ---cCGCGCGUGGucGUCGGCGug-GCCg -3' miRNA: 3'- uuauGCGCGUACU--UAGCCGUuuaCGG- -5' |
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| 26703 | 3' | -48.2 | NC_005808.1 | + | 18629 | 1.12 | 0.002157 |
|
Target: 5'- gAAUACGCGCAUGAAUCGGCAAAUGCCg -3' miRNA: 3'- -UUAUGCGCGUACUUAGCCGUUUACGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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