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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 26703 | 3' | -48.2 | NC_005808.1 | + | 22535 | 0.66 | 0.94131 |
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Target: 5'- --cACGCGCAg----CGcGCGcGUGCCg -3' miRNA: 3'- uuaUGCGCGUacuuaGC-CGUuUACGG- -5' |
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| 26703 | 3' | -48.2 | NC_005808.1 | + | 24115 | 0.66 | 0.94131 |
|
Target: 5'- cAUGCGCGCGUagGggUCGuaGuccaGCCg -3' miRNA: 3'- uUAUGCGCGUA--CuuAGCcgUuua-CGG- -5' |
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| 26703 | 3' | -48.2 | NC_005808.1 | + | 41222 | 0.66 | 0.94131 |
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Target: 5'- ---gUGCGCGUGGccgCGGCGcccuAUGUCa -3' miRNA: 3'- uuauGCGCGUACUua-GCCGUu---UACGG- -5' |
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| 26703 | 3' | -48.2 | NC_005808.1 | + | 13471 | 0.66 | 0.946556 |
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Target: 5'- cGGUGuCGgGCAUGAccUgGGCG-AUGCCg -3' miRNA: 3'- -UUAU-GCgCGUACUu-AgCCGUuUACGG- -5' |
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| 26703 | 3' | -48.2 | NC_005808.1 | + | 31449 | 0.66 | 0.951483 |
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Target: 5'- -cUGCG-GCGUGGucAUCGGaaaguGGGUGCCu -3' miRNA: 3'- uuAUGCgCGUACU--UAGCCg----UUUACGG- -5' |
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| 26703 | 3' | -48.2 | NC_005808.1 | + | 28775 | 0.66 | 0.951483 |
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Target: 5'- --gGCGCGCAcgGcGUCGcGCu--UGCCc -3' miRNA: 3'- uuaUGCGCGUa-CuUAGC-CGuuuACGG- -5' |
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| 26703 | 3' | -48.2 | NC_005808.1 | + | 24878 | 0.66 | 0.951483 |
|
Target: 5'- aAAUGCGCGCAgcuUGccgcGAUgGGCAuuucccgGCCc -3' miRNA: 3'- -UUAUGCGCGU---AC----UUAgCCGUuua----CGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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