Results 61 - 67 of 67 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26703 | 3' | -48.2 | NC_005808.1 | + | 4371 | 0.74 | 0.561772 |
Target: 5'- ---gUGCGCGUGAa--GGCGAcgGCCg -3' miRNA: 3'- uuauGCGCGUACUuagCCGUUuaCGG- -5' |
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26703 | 3' | -48.2 | NC_005808.1 | + | 4192 | 0.69 | 0.824785 |
Target: 5'- cAGUGgGCGCcgaaaaguugGAAUCGGCGcgcaccGUGCCa -3' miRNA: 3'- -UUAUgCGCGua--------CUUAGCCGUu-----UACGG- -5' |
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26703 | 3' | -48.2 | NC_005808.1 | + | 3272 | 0.67 | 0.910216 |
Target: 5'- ---cCGCGCcaGAuaGUCGGCccGUGCCu -3' miRNA: 3'- uuauGCGCGuaCU--UAGCCGuuUACGG- -5' |
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26703 | 3' | -48.2 | NC_005808.1 | + | 2231 | 0.68 | 0.895515 |
Target: 5'- --cACGCGCAgcaggCGGCGcGUGgCCg -3' miRNA: 3'- uuaUGCGCGUacuuaGCCGUuUAC-GG- -5' |
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26703 | 3' | -48.2 | NC_005808.1 | + | 638 | 0.66 | 0.935742 |
Target: 5'- --gGCGCGgGUGAacggGUCaGGCGGggGCUu -3' miRNA: 3'- uuaUGCGCgUACU----UAG-CCGUUuaCGG- -5' |
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26703 | 3' | -48.2 | NC_005808.1 | + | 468 | 0.67 | 0.903025 |
Target: 5'- -uUGCGgGCGcUGucgCGGuCGGAUGCCu -3' miRNA: 3'- uuAUGCgCGU-ACuuaGCC-GUUUACGG- -5' |
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26703 | 3' | -48.2 | NC_005808.1 | + | 177 | 0.66 | 0.951483 |
Target: 5'- --cGCGCGCA-GAAUgcCGGCGucauccccGCCa -3' miRNA: 3'- uuaUGCGCGUaCUUA--GCCGUuua-----CGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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