Results 61 - 67 of 67 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 26703 | 3' | -48.2 | NC_005808.1 | + | 6313 | 0.7 | 0.761471 |
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Target: 5'- cGGUGCGUGCAaGAcgCGGCGcGUuccGCCc -3' miRNA: 3'- -UUAUGCGCGUaCUuaGCCGUuUA---CGG- -5' |
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| 26703 | 3' | -48.2 | NC_005808.1 | + | 16328 | 0.69 | 0.814753 |
|
Target: 5'- cGAUGCGCGagccGAccaggcCGGCGGGUGCCc -3' miRNA: 3'- -UUAUGCGCgua-CUua----GCCGUUUACGG- -5' |
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| 26703 | 3' | -48.2 | NC_005808.1 | + | 29313 | 0.69 | 0.853401 |
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Target: 5'- --gGCGCGC-UGcAAUCGGCGcugaaaGAcGCCa -3' miRNA: 3'- uuaUGCGCGuAC-UUAGCCGU------UUaCGG- -5' |
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| 26703 | 3' | -48.2 | NC_005808.1 | + | 34941 | 0.68 | 0.862407 |
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Target: 5'- --aACgGCGCGUGua-CGGCAAGgaGCCg -3' miRNA: 3'- uuaUG-CGCGUACuuaGCCGUUUa-CGG- -5' |
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| 26703 | 3' | -48.2 | NC_005808.1 | + | 13485 | 0.68 | 0.863292 |
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Target: 5'- --cGCGCGCGUGAc-CGGCAGcaagucccgcacggGCCu -3' miRNA: 3'- uuaUGCGCGUACUuaGCCGUUua------------CGG- -5' |
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| 26703 | 3' | -48.2 | NC_005808.1 | + | 10476 | 0.68 | 0.87113 |
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Target: 5'- --cGgGgGCGUcGAgGUCGGCAGcgGCCa -3' miRNA: 3'- uuaUgCgCGUA-CU-UAGCCGUUuaCGG- -5' |
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| 26703 | 3' | -48.2 | NC_005808.1 | + | 18629 | 1.12 | 0.002157 |
|
Target: 5'- gAAUACGCGCAUGAAUCGGCAAAUGCCg -3' miRNA: 3'- -UUAUGCGCGUACUUAGCCGUUUACGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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