Results 61 - 67 of 67 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 26703 | 3' | -48.2 | NC_005808.1 | + | 13017 | 0.67 | 0.917086 |
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Target: 5'- ---cCGCGCAgcgcagUGGCGAcgGCCu -3' miRNA: 3'- uuauGCGCGUacuua-GCCGUUuaCGG- -5' |
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| 26703 | 3' | -48.2 | NC_005808.1 | + | 38893 | 0.67 | 0.910216 |
|
Target: 5'- gGAUGCGcCGCAUGGggCaGCGcGUcGCCg -3' miRNA: 3'- -UUAUGC-GCGUACUuaGcCGUuUA-CGG- -5' |
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| 26703 | 3' | -48.2 | NC_005808.1 | + | 19238 | 0.67 | 0.910216 |
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Target: 5'- --aGCGCGgcgucaggccCAUGAAcuucUCGGCGucGAUGCUg -3' miRNA: 3'- uuaUGCGC----------GUACUU----AGCCGU--UUACGG- -5' |
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| 26703 | 3' | -48.2 | NC_005808.1 | + | 15391 | 0.67 | 0.903025 |
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Target: 5'- --cGCGUGCGggc--CGGCGaaGAUGCCg -3' miRNA: 3'- uuaUGCGCGUacuuaGCCGU--UUACGG- -5' |
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| 26703 | 3' | -48.2 | NC_005808.1 | + | 468 | 0.67 | 0.903025 |
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Target: 5'- -uUGCGgGCGcUGucgCGGuCGGAUGCCu -3' miRNA: 3'- uuAUGCgCGU-ACuuaGCC-GUUUACGG- -5' |
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| 26703 | 3' | -48.2 | NC_005808.1 | + | 2231 | 0.68 | 0.895515 |
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Target: 5'- --cACGCGCAgcaggCGGCGcGUGgCCg -3' miRNA: 3'- uuaUGCGCGUacuuaGCCGUuUAC-GG- -5' |
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| 26703 | 3' | -48.2 | NC_005808.1 | + | 12368 | 0.75 | 0.481697 |
|
Target: 5'- gGAUuuGCGCGUcGGUCGGCGagucGAUGCCu -3' miRNA: 3'- -UUAugCGCGUAcUUAGCCGU----UUACGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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