Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26703 | 5' | -60.8 | NC_005808.1 | + | 4375 | 0.67 | 0.320324 |
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Target: 5'- -aGGCGgaUGCGCagccagGcGCCGUCGCGCCg -3' miRNA: 3'- gcCCGC--ACGCGga----C-UGGUAGUGCGGa -5' |
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| 26703 | 5' | -60.8 | NC_005808.1 | + | 32866 | 0.67 | 0.328117 |
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Target: 5'- aCGGuCG-GCGCCgaGAUUGUCGCGCCc -3' miRNA: 3'- -GCCcGCaCGCGGa-CUGGUAGUGCGGa -5' |
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| 26703 | 5' | -60.8 | NC_005808.1 | + | 40148 | 0.66 | 0.33605 |
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Target: 5'- -aGGCGaGCGCCUgGGCCGcaguuUCgGCGCCUu -3' miRNA: 3'- gcCCGCaCGCGGA-CUGGU-----AG-UGCGGA- -5' |
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| 26703 | 5' | -60.8 | NC_005808.1 | + | 34450 | 0.66 | 0.352332 |
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Target: 5'- aGGGaCGcaGCGCCgacACCcgCACGCCc -3' miRNA: 3'- gCCC-GCa-CGCGGac-UGGuaGUGCGGa -5' |
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| 26703 | 5' | -60.8 | NC_005808.1 | + | 18268 | 0.66 | 0.352332 |
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Target: 5'- aCGGGCaucgcucUGcCGCCUGACCcgCGgcugcuCGCCg -3' miRNA: 3'- -GCCCGc------AC-GCGGACUGGuaGU------GCGGa -5' |
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| 26703 | 5' | -60.8 | NC_005808.1 | + | 14679 | 0.66 | 0.352332 |
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Target: 5'- gCGGGUGUugaGCGCUUGaaGCCcggccugcaGUUGCGCCUc -3' miRNA: 3'- -GCCCGCA---CGCGGAC--UGG---------UAGUGCGGA- -5' |
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| 26703 | 5' | -60.8 | NC_005808.1 | + | 39705 | 0.66 | 0.360681 |
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Target: 5'- gGGGCGcgcgGCGUCUGugcCCAUCAC-CUUc -3' miRNA: 3'- gCCCGCa---CGCGGACu--GGUAGUGcGGA- -5' |
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| 26703 | 5' | -60.8 | NC_005808.1 | + | 25996 | 0.66 | 0.360681 |
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Target: 5'- -cGGCG-GCGCCaacACCAUCGCGUg- -3' miRNA: 3'- gcCCGCaCGCGGac-UGGUAGUGCGga -5' |
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| 26703 | 5' | -60.8 | NC_005808.1 | + | 28197 | 0.66 | 0.377787 |
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Target: 5'- aGGuGCG-GCGCCuUGuCCAggucggcCACGCCg -3' miRNA: 3'- gCC-CGCaCGCGG-ACuGGUa------GUGCGGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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