Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26705 | 3' | -56.6 | NC_005808.1 | + | 27599 | 0.66 | 0.572301 |
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Target: 5'- gGCGCCAgauggcGUGGUcgucgcccUUGACGauggGCCGgUGg -3' miRNA: 3'- -UGCGGUa-----CACCA--------AGCUGCa---CGGCgAC- -5' |
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| 26705 | 3' | -56.6 | NC_005808.1 | + | 35518 | 0.66 | 0.538481 |
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Target: 5'- cGCGCCGUG-GGUaggccgCGGCGUcaugaaguaccugGCCGCc- -3' miRNA: 3'- -UGCGGUACaCCAa-----GCUGCA-------------CGGCGac -5' |
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| 26705 | 3' | -56.6 | NC_005808.1 | + | 15345 | 0.67 | 0.507481 |
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Target: 5'- -aGCCGUagGGUUCgGACGUG-CGCUGc -3' miRNA: 3'- ugCGGUAcaCCAAG-CUGCACgGCGAC- -5' |
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| 26705 | 3' | -56.6 | NC_005808.1 | + | 39526 | 0.67 | 0.506426 |
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Target: 5'- -gGgCAUG-GGUgcggcucgggcgcUUGACGUGCUGCUGa -3' miRNA: 3'- ugCgGUACaCCA-------------AGCUGCACGGCGAC- -5' |
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| 26705 | 3' | -56.6 | NC_005808.1 | + | 32794 | 0.68 | 0.44598 |
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Target: 5'- aACGCUgcuUGacUGGUUCGGCGUGCgCGacCUGg -3' miRNA: 3'- -UGCGGu--AC--ACCAAGCUGCACG-GC--GAC- -5' |
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| 26705 | 3' | -56.6 | NC_005808.1 | + | 1707 | 0.68 | 0.426415 |
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Target: 5'- uCGCCGUG-GGc-CGGCGUGCCGg-- -3' miRNA: 3'- uGCGGUACaCCaaGCUGCACGGCgac -5' |
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| 26705 | 3' | -56.6 | NC_005808.1 | + | 5808 | 0.68 | 0.426415 |
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Target: 5'- aGCGCCAgcggauaGUcGGgcaUGGCGUGCCGCa- -3' miRNA: 3'- -UGCGGUa------CA-CCaa-GCUGCACGGCGac -5' |
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| 26705 | 3' | -56.6 | NC_005808.1 | + | 3738 | 0.72 | 0.268503 |
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Target: 5'- cACGCCGgugGUGGgcacgUCGGUGccGCCGCUGg -3' miRNA: 3'- -UGCGGUa--CACCa----AGCUGCa-CGGCGAC- -5' |
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| 26705 | 3' | -56.6 | NC_005808.1 | + | 17974 | 1.08 | 0.000592 |
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Target: 5'- cACGCCAUGUGGUUCGACGUGCCGCUGa -3' miRNA: 3'- -UGCGGUACACCAAGCUGCACGGCGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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