miRNA: 3'- -UGAACACCAACAGCAGCUCCGCCAUG- -5'
miRNA: 3'- -UGAaCACCaACAGCAGCu----CCGCcAUG- -5'
miRNA: 3'- -UGAAC---ACCAACaGCA------------GCUCCGCCAug -5'
Results 1 - 3 of 3 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 26718 | 3' | -53 | NC_005808.1 | + | 8800 | 1.09 | 0.001168 |
|
Target: 5'- aACUUGUGGUUGUCGUCGAGGCGGUACu -3' miRNA: 3'- -UGAACACCAACAGCAGCUCCGCCAUG- -5' |
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| 26718 | 3' | -53 | NC_005808.1 | + | 12890 | 0.69 | 0.599088 |
|
Target: 5'- gGCUcGUGG-UGUCGUCGccuuuGGCGaUGCg -3' miRNA: 3'- -UGAaCACCaACAGCAGCu----CCGCcAUG- -5' |
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| 26718 | 3' | -53 | NC_005808.1 | + | 19742 | 0.69 | 0.576454 |
|
Target: 5'- uGCUUGggcUGGUUGcCGUuguugccggccaCGAGGCGGUc- -3' miRNA: 3'- -UGAAC---ACCAACaGCA------------GCUCCGCCAug -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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