Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2672 | 5' | -50.7 | NC_001491.2 | + | 93219 | 0.66 | 0.992171 |
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Target: 5'- cGUCUCgUUGCGAcCCCCAaagguGCCAGc--- -3' miRNA: 3'- -CAGAG-AGCGUUuGGGGU-----CGGUUauuc -5' |
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| 2672 | 5' | -50.7 | NC_001491.2 | + | 117061 | 0.66 | 0.990998 |
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Target: 5'- -gCUCUCGCGcGCCCCAaGCa------ -3' miRNA: 3'- caGAGAGCGUuUGGGGU-CGguuauuc -5' |
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| 2672 | 5' | -50.7 | NC_001491.2 | + | 12971 | 0.66 | 0.989692 |
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Target: 5'- aGUCUg-CGCuuGCCCCGGuCCGAgcAGc -3' miRNA: 3'- -CAGAgaGCGuuUGGGGUC-GGUUauUC- -5' |
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| 2672 | 5' | -50.7 | NC_001491.2 | + | 34940 | 0.66 | 0.988241 |
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Target: 5'- cUCUCggCGCGAGCCCCucuGGuCCGAc--- -3' miRNA: 3'- cAGAGa-GCGUUUGGGG---UC-GGUUauuc -5' |
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| 2672 | 5' | -50.7 | NC_001491.2 | + | 116752 | 0.67 | 0.986638 |
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Target: 5'- gGUUUCUC-CGAGCagCCGGCCAGaGAGu -3' miRNA: 3'- -CAGAGAGcGUUUGg-GGUCGGUUaUUC- -5' |
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| 2672 | 5' | -50.7 | NC_001491.2 | + | 114344 | 0.67 | 0.986638 |
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Target: 5'- -aCUCUUGCAGuGCCUCuGCCGccGAGg -3' miRNA: 3'- caGAGAGCGUU-UGGGGuCGGUuaUUC- -5' |
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| 2672 | 5' | -50.7 | NC_001491.2 | + | 44710 | 0.67 | 0.984871 |
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Target: 5'- uGUCUggCUCGU--AUCCCAGCUuauAGUAAGg -3' miRNA: 3'- -CAGA--GAGCGuuUGGGGUCGG---UUAUUC- -5' |
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| 2672 | 5' | -50.7 | NC_001491.2 | + | 146551 | 0.68 | 0.970331 |
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Target: 5'- -aCUCUuCGCGGGCCCUuGCCAu---- -3' miRNA: 3'- caGAGA-GCGUUUGGGGuCGGUuauuc -5' |
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| 2672 | 5' | -50.7 | NC_001491.2 | + | 73821 | 1.09 | 0.007843 |
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Target: 5'- cGUCUCUCGCAAACCCCAGCCAAUAAGu -3' miRNA: 3'- -CAGAGAGCGUUUGGGGUCGGUUAUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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