miRNA: 3'- uACCGGaa-CGUGGUCCUGAGcu--GGUC- -5'
miRNA: 3'- uaCCGga----ACGUGGUCCUG-AGCUGGUc -5'
miRNA: 3'- uaCCG-GA------ACGUGGUCC---------UGAGCUGGuc -5'
miRNA: 3'- uaCCGGAACGUGGu-------CCUGAGC------UGGUc -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 26720 | 5' | -57.6 | NC_005808.1 | + | 6287 | 0.7 | 0.263015 |
|
Target: 5'- uUGGCUgggGCGCCGGGGCUUaucuCCGGu -3' miRNA: 3'- uACCGGaa-CGUGGUCCUGAGcu--GGUC- -5' |
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| 26720 | 5' | -57.6 | NC_005808.1 | + | 5759 | 0.73 | 0.170896 |
|
Target: 5'- -gGGCagcgagUGCGCCAGGGCcUUGACCAu -3' miRNA: 3'- uaCCGga----ACGUGGUCCUG-AGCUGGUc -5' |
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| 26720 | 5' | -57.6 | NC_005808.1 | + | 4576 | 0.67 | 0.391637 |
|
Target: 5'- -aGGCgCUgcucggUGCGCCAGGcgaggaacuGCUCGGCCu- -3' miRNA: 3'- uaCCG-GA------ACGUGGUCC---------UGAGCUGGuc -5' |
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| 26720 | 5' | -57.6 | NC_005808.1 | + | 1666 | 0.71 | 0.230529 |
|
Target: 5'- -aGGUCUUGCACCucgcgcuuGGACUCGcggcgaGCCAu -3' miRNA: 3'- uaCCGGAACGUGGu-------CCUGAGC------UGGUc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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