Results 21 - 31 of 31 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26724 | 3' | -60.3 | NC_005808.1 | + | 18858 | 0.67 | 0.325077 |
Target: 5'- aGgCCGGGCg--GCCCAUGCCCacgUUCGGg -3' miRNA: 3'- -CgGGCUCGgcgUGGGUACGGG---AAGCU- -5' |
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26724 | 3' | -60.3 | NC_005808.1 | + | 6339 | 0.67 | 0.332967 |
Target: 5'- cGCCCcAGCCaaaGCGCCCG-GCCCgaaggcCGGg -3' miRNA: 3'- -CGGGcUCGG---CGUGGGUaCGGGaa----GCU- -5' |
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26724 | 3' | -60.3 | NC_005808.1 | + | 11355 | 0.68 | 0.302247 |
Target: 5'- cGCCCGgcucguagcugGGCCGCGCgCCGggGUUCUUCa- -3' miRNA: 3'- -CGGGC-----------UCGGCGUG-GGUa-CGGGAAGcu -5' |
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26724 | 3' | -60.3 | NC_005808.1 | + | 16331 | 0.69 | 0.233309 |
Target: 5'- uGCgCGAGCCgaccaggccggcggGUGCCCGUGCCUUcggCGAc -3' miRNA: 3'- -CGgGCUCGG--------------CGUGGGUACGGGAa--GCU- -5' |
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26724 | 3' | -60.3 | NC_005808.1 | + | 12919 | 0.69 | 0.253833 |
Target: 5'- cGCUCG-GCCGCGCCCAUaacgcuagucaGCUUcUCGGg -3' miRNA: 3'- -CGGGCuCGGCGUGGGUA-----------CGGGaAGCU- -5' |
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26724 | 3' | -60.3 | NC_005808.1 | + | 40155 | 0.7 | 0.21757 |
Target: 5'- cGCCUGGGCCGCAgUUUcgGCgCCUUCa- -3' miRNA: 3'- -CGGGCUCGGCGU-GGGuaCG-GGAAGcu -5' |
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26724 | 3' | -60.3 | NC_005808.1 | + | 14703 | 0.7 | 0.21757 |
Target: 5'- gGCCUGcAGuuGCGCcuCCAUGCCCUgCGc -3' miRNA: 3'- -CGGGC-UCggCGUG--GGUACGGGAaGCu -5' |
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26724 | 3' | -60.3 | NC_005808.1 | + | 24912 | 0.71 | 0.185831 |
Target: 5'- gGCCCGAGUgGCgcuucgccuacGCCCA-GCCCgcCGAc -3' miRNA: 3'- -CGGGCUCGgCG-----------UGGGUaCGGGaaGCU- -5' |
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26724 | 3' | -60.3 | NC_005808.1 | + | 38030 | 0.71 | 0.185831 |
Target: 5'- cGCaCCGAGCaGCGCCUcgGCgaCUUCGGc -3' miRNA: 3'- -CG-GGCUCGgCGUGGGuaCGg-GAAGCU- -5' |
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26724 | 3' | -60.3 | NC_005808.1 | + | 802 | 0.73 | 0.134414 |
Target: 5'- uUCCGGcgacgcGCCGCGCCCA-GCCCUUgcCGAa -3' miRNA: 3'- cGGGCU------CGGCGUGGGUaCGGGAA--GCU- -5' |
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26724 | 3' | -60.3 | NC_005808.1 | + | 3075 | 1.1 | 0.000196 |
Target: 5'- cGCCCGAGCCGCACCCAUGCCCUUCGAc -3' miRNA: 3'- -CGGGCUCGGCGUGGGUACGGGAAGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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