Results 1 - 20 of 39 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26726 | 3' | -54.1 | NC_005808.1 | + | 2046 | 1.14 | 0.000541 |
Target: 5'- gCCUCGGCGACGAUGGAUUCAAGGGCCu -3' miRNA: 3'- -GGAGCCGCUGCUACCUAAGUUCCCGG- -5' |
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26726 | 3' | -54.1 | NC_005808.1 | + | 4612 | 0.81 | 0.115104 |
Target: 5'- gCCUCGGCGAUGAUgucggcggGGAUgcu-GGGCCa -3' miRNA: 3'- -GGAGCCGCUGCUA--------CCUAaguuCCCGG- -5' |
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26726 | 3' | -54.1 | NC_005808.1 | + | 7594 | 0.74 | 0.289934 |
Target: 5'- uCgUCGGCGACGGUGGAcagCAcGuGGUCg -3' miRNA: 3'- -GgAGCCGCUGCUACCUaa-GUuC-CCGG- -5' |
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26726 | 3' | -54.1 | NC_005808.1 | + | 10458 | 0.73 | 0.345082 |
Target: 5'- gUUCGGCcuUGAgGGAUUCGGGGGCg -3' miRNA: 3'- gGAGCCGcuGCUaCCUAAGUUCCCGg -5' |
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26726 | 3' | -54.1 | NC_005808.1 | + | 633 | 0.73 | 0.345082 |
Target: 5'- gCCUCGGCG-CGGgugaacGGGUcaggCGGGGGCUu -3' miRNA: 3'- -GGAGCCGCuGCUa-----CCUAa---GUUCCCGG- -5' |
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26726 | 3' | -54.1 | NC_005808.1 | + | 11385 | 0.73 | 0.353549 |
Target: 5'- aCCUCGGUGGCGAccugaUGGg--CcAGGGUCu -3' miRNA: 3'- -GGAGCCGCUGCU-----ACCuaaGuUCCCGG- -5' |
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26726 | 3' | -54.1 | NC_005808.1 | + | 17281 | 0.73 | 0.37092 |
Target: 5'- uCC-CGGCGACGAgcgccGGuggC-AGGGCCg -3' miRNA: 3'- -GGaGCCGCUGCUa----CCuaaGuUCCCGG- -5' |
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26726 | 3' | -54.1 | NC_005808.1 | + | 25988 | 0.72 | 0.387954 |
Target: 5'- uCCUCGccggucuGCGGCGuauUGGcgauUUCGAGGGCUu -3' miRNA: 3'- -GGAGC-------CGCUGCu--ACCu---AAGUUCCCGG- -5' |
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26726 | 3' | -54.1 | NC_005808.1 | + | 22247 | 0.71 | 0.426415 |
Target: 5'- -gUCGGCcGCGAUGGcguAUUCGccGGCCg -3' miRNA: 3'- ggAGCCGcUGCUACC---UAAGUucCCGG- -5' |
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26726 | 3' | -54.1 | NC_005808.1 | + | 10242 | 0.71 | 0.455949 |
Target: 5'- gCCUCGGCGGCGAccgcagcgaccUGGGcggcgguugUCGGGacgccGGCCu -3' miRNA: 3'- -GGAGCCGCUGCU-----------ACCUa--------AGUUC-----CCGG- -5' |
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26726 | 3' | -54.1 | NC_005808.1 | + | 26187 | 0.71 | 0.455949 |
Target: 5'- uUUCGGUGGCGAUGGcUUCGGuguaGGUCg -3' miRNA: 3'- gGAGCCGCUGCUACCuAAGUUc---CCGG- -5' |
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26726 | 3' | -54.1 | NC_005808.1 | + | 6842 | 0.71 | 0.455949 |
Target: 5'- cCCUCGGCGACGGccuuUUCGuaGGCCc -3' miRNA: 3'- -GGAGCCGCUGCUaccuAAGUucCCGG- -5' |
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26726 | 3' | -54.1 | NC_005808.1 | + | 7144 | 0.7 | 0.48655 |
Target: 5'- uCgUCGGCuGCGGccUGGAUgaccAGGGCCg -3' miRNA: 3'- -GgAGCCGcUGCU--ACCUAagu-UCCCGG- -5' |
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26726 | 3' | -54.1 | NC_005808.1 | + | 28053 | 0.7 | 0.49592 |
Target: 5'- aCgUCGGCGACGGUcugcucgaacagcGGg--CAGGuGGCCg -3' miRNA: 3'- -GgAGCCGCUGCUA-------------CCuaaGUUC-CCGG- -5' |
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26726 | 3' | -54.1 | NC_005808.1 | + | 34693 | 0.7 | 0.507481 |
Target: 5'- cCCUCGGCaa-GAUGGGaggcaAAGGGCa -3' miRNA: 3'- -GGAGCCGcugCUACCUaag--UUCCCGg -5' |
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26726 | 3' | -54.1 | NC_005808.1 | + | 30420 | 0.7 | 0.528786 |
Target: 5'- gCUCGGCauGGCGAUGagcgCGggcAGGGCCu -3' miRNA: 3'- gGAGCCG--CUGCUACcuaaGU---UCCCGG- -5' |
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26726 | 3' | -54.1 | NC_005808.1 | + | 12544 | 0.69 | 0.550412 |
Target: 5'- aCCUCGGCGuCGGUGaauUUCuugccGGCCu -3' miRNA: 3'- -GGAGCCGCuGCUACcu-AAGuuc--CCGG- -5' |
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26726 | 3' | -54.1 | NC_005808.1 | + | 36456 | 0.69 | 0.561327 |
Target: 5'- --aCGGCGGCGAgUGGGUgcugauggAAGGGCa -3' miRNA: 3'- ggaGCCGCUGCU-ACCUAag------UUCCCGg -5' |
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26726 | 3' | -54.1 | NC_005808.1 | + | 10769 | 0.69 | 0.583326 |
Target: 5'- uCgUCGGUGAgGGUGcgcaGGUUCAuGGGCUu -3' miRNA: 3'- -GgAGCCGCUgCUAC----CUAAGUuCCCGG- -5' |
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26726 | 3' | -54.1 | NC_005808.1 | + | 8447 | 0.68 | 0.616612 |
Target: 5'- -aUCGGCG-CGAUgucGGcgUCGAGGugGCCu -3' miRNA: 3'- ggAGCCGCuGCUA---CCuaAGUUCC--CGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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