Results 1 - 20 of 21 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26726 | 5' | -60.6 | NC_005808.1 | + | 3418 | 0.69 | 0.236393 |
Target: 5'- -gGGGCcuuUCGGCCCcgcuacucaagaaucAGGUGGUcacagaCGCCGc -3' miRNA: 3'- caCCUGu--AGCCGGG---------------UCCACCA------GCGGC- -5' |
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26726 | 5' | -60.6 | NC_005808.1 | + | 11569 | 0.66 | 0.398804 |
Target: 5'- -cGGuCAUCGaagccaugccGCCCAGGgccgccggcUGGcCGCCGg -3' miRNA: 3'- caCCuGUAGC----------CGGGUCC---------ACCaGCGGC- -5' |
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26726 | 5' | -60.6 | NC_005808.1 | + | 32341 | 0.66 | 0.389879 |
Target: 5'- --cGACAaccgCcGCCCAGGUcgcugcGGUCGCCGc -3' miRNA: 3'- cacCUGUa---GcCGGGUCCA------CCAGCGGC- -5' |
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26726 | 5' | -60.6 | NC_005808.1 | + | 19954 | 0.66 | 0.381086 |
Target: 5'- cGUGGACAggcaaCCCAGGUuucgggcaGGcCGCCGa -3' miRNA: 3'- -CACCUGUagcc-GGGUCCA--------CCaGCGGC- -5' |
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26726 | 5' | -60.6 | NC_005808.1 | + | 8606 | 0.66 | 0.367294 |
Target: 5'- cUGGAUAagcugguugcgcuucUCGGCCgCugcGGUggcgaGGUCGCCGg -3' miRNA: 3'- cACCUGU---------------AGCCGG-Gu--CCA-----CCAGCGGC- -5' |
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26726 | 5' | -60.6 | NC_005808.1 | + | 35032 | 0.67 | 0.339145 |
Target: 5'- cGUGGACGU--GUCCAagccGGUGG-CGCCGc -3' miRNA: 3'- -CACCUGUAgcCGGGU----CCACCaGCGGC- -5' |
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26726 | 5' | -60.6 | NC_005808.1 | + | 18402 | 0.67 | 0.308077 |
Target: 5'- gGUGGcggccACGUCGGCgaAGGUGGcguugucCGCCGa -3' miRNA: 3'- -CACC-----UGUAGCCGggUCCACCa------GCGGC- -5' |
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26726 | 5' | -60.6 | NC_005808.1 | + | 3964 | 0.67 | 0.308077 |
Target: 5'- cUGGugcCAUCGG-UCAGGUGGUugaCGCCa -3' miRNA: 3'- cACCu--GUAGCCgGGUCCACCA---GCGGc -5' |
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26726 | 5' | -60.6 | NC_005808.1 | + | 5523 | 0.68 | 0.286234 |
Target: 5'- -aGGACA-CGccggccGCCCAGGUuccgcccaGGUCGCCc -3' miRNA: 3'- caCCUGUaGC------CGGGUCCA--------CCAGCGGc -5' |
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26726 | 5' | -60.6 | NC_005808.1 | + | 16418 | 0.69 | 0.240048 |
Target: 5'- -aGGACA-CGGCCgCGGGguaaUCGCCGg -3' miRNA: 3'- caCCUGUaGCCGG-GUCCacc-AGCGGC- -5' |
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26726 | 5' | -60.6 | NC_005808.1 | + | 27637 | 0.71 | 0.177332 |
Target: 5'- -cGGugGUUGGucggcaugaaagcuaCCCAGGUGGgCGCCa -3' miRNA: 3'- caCCugUAGCC---------------GGGUCCACCaGCGGc -5' |
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26726 | 5' | -60.6 | NC_005808.1 | + | 2086 | 1.08 | 0.000275 |
Target: 5'- cGUGGACAUCGGCCCAGGUGGUCGCCGu -3' miRNA: 3'- -CACCUGUAGCCGGGUCCACCAGCGGC- -5' |
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26726 | 5' | -60.6 | NC_005808.1 | + | 18837 | 0.8 | 0.038691 |
Target: 5'- -cGGcGCAUCGGCCC-GGUGG-CGCCGg -3' miRNA: 3'- caCC-UGUAGCCGGGuCCACCaGCGGC- -5' |
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26726 | 5' | -60.6 | NC_005808.1 | + | 24899 | 0.7 | 0.200253 |
Target: 5'- aUGGGCAUuuccCGGCCCGaGUGGcgcuUCGCCu -3' miRNA: 3'- cACCUGUA----GCCGGGUcCACC----AGCGGc -5' |
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26726 | 5' | -60.6 | NC_005808.1 | + | 39766 | 0.68 | 0.259035 |
Target: 5'- -cGGGC--UGGCCUuuccucaauAGGUGGUCaGCCGg -3' miRNA: 3'- caCCUGuaGCCGGG---------UCCACCAG-CGGC- -5' |
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26726 | 5' | -60.6 | NC_005808.1 | + | 17589 | 0.68 | 0.262976 |
Target: 5'- -cGGACggcaacccgcugucGUCGGCCCccuuugugcugguGGGUGGgcgCGUCGa -3' miRNA: 3'- caCCUG--------------UAGCCGGG-------------UCCACCa--GCGGC- -5' |
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26726 | 5' | -60.6 | NC_005808.1 | + | 35745 | 0.67 | 0.331169 |
Target: 5'- cGUGGugGggGGCCUacgaaaAGGccGUCGCCGa -3' miRNA: 3'- -CACCugUagCCGGG------UCCacCAGCGGC- -5' |
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26726 | 5' | -60.6 | NC_005808.1 | + | 26769 | 0.66 | 0.372425 |
Target: 5'- -cGGACGgcguguUCGaGUCCugcgcGGUGGUgGCCGa -3' miRNA: 3'- caCCUGU------AGC-CGGGu----CCACCAgCGGC- -5' |
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26726 | 5' | -60.6 | NC_005808.1 | + | 12750 | 0.66 | 0.381086 |
Target: 5'- -aGGAag-CGGCacaaCAGG-GGUCGCUGc -3' miRNA: 3'- caCCUguaGCCGg---GUCCaCCAGCGGC- -5' |
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26726 | 5' | -60.6 | NC_005808.1 | + | 4060 | 0.66 | 0.381086 |
Target: 5'- -aGGcCAgCGGCCCAGGggggauUGGUaGCCa -3' miRNA: 3'- caCCuGUaGCCGGGUCC------ACCAgCGGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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