Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 26739 | 3' | -52.2 | NC_005808.1 | + | 24666 | 0.66 | 0.819304 |
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Target: 5'- uGUUGUc-GCGCCCGcc-GCGGGCCa -3' miRNA: 3'- gCAGCAacUGCGGGCuuaUGUCUGGc -5' |
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| 26739 | 3' | -52.2 | NC_005808.1 | + | 29965 | 0.69 | 0.693972 |
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Target: 5'- uCGUCGcggcgcacGACgGCCUGGAUGCAG-CCGu -3' miRNA: 3'- -GCAGCaa------CUG-CGGGCUUAUGUCuGGC- -5' |
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| 26739 | 3' | -52.2 | NC_005808.1 | + | 32486 | 0.66 | 0.828682 |
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Target: 5'- gGcCGaUG-CGCCCGAAUccaAGGCCGa -3' miRNA: 3'- gCaGCaACuGCGGGCUUAug-UCUGGC- -5' |
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| 26739 | 3' | -52.2 | NC_005808.1 | + | 32999 | 0.68 | 0.759091 |
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Target: 5'- aCGUCGaacucACGCCCGAgGUGCGcaacguguucGACCGg -3' miRNA: 3'- -GCAGCaac--UGCGGGCU-UAUGU----------CUGGC- -5' |
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| 26739 | 3' | -52.2 | NC_005808.1 | + | 38982 | 0.68 | 0.705053 |
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Target: 5'- gCGgCGUgaaUGGCGCauCCGAAUACAcGGCCa -3' miRNA: 3'- -GCaGCA---ACUGCG--GGCUUAUGU-CUGGc -5' |
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| 26739 | 3' | -52.2 | NC_005808.1 | + | 39852 | 0.71 | 0.537309 |
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Target: 5'- gGUCGgc-ACGCCCGA--GCAGcGCCGg -3' miRNA: 3'- gCAGCaacUGCGGGCUuaUGUC-UGGC- -5' |
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| 26739 | 3' | -52.2 | NC_005808.1 | + | 40228 | 0.66 | 0.855472 |
|
Target: 5'- --aCGcgGGCGUCUGccacGUGCAGGCCGa -3' miRNA: 3'- gcaGCaaCUGCGGGCu---UAUGUCUGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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