miRNA display CGI


Results 21 - 27 of 27 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
26739 3' -52.2 NC_005808.1 + 32999 0.68 0.759091
Target:  5'- aCGUCGaacucACGCCCGAgGUGCGcaacguguucGACCGg -3'
miRNA:   3'- -GCAGCaac--UGCGGGCU-UAUGU----------CUGGC- -5'
26739 3' -52.2 NC_005808.1 + 17000 0.68 0.759091
Target:  5'- cCGUgGUUGccacUGCCUGGGccGCAGACCGc -3'
miRNA:   3'- -GCAgCAACu---GCGGGCUUa-UGUCUGGC- -5'
26739 3' -52.2 NC_005808.1 + 38982 0.68 0.705053
Target:  5'- gCGgCGUgaaUGGCGCauCCGAAUACAcGGCCa -3'
miRNA:   3'- -GCaGCA---ACUGCG--GGCUUAUGU-CUGGc -5'
26739 3' -52.2 NC_005808.1 + 2721 0.69 0.682828
Target:  5'- aCGUUGgccgGACGCuuGAGcGCGGcCCGg -3'
miRNA:   3'- -GCAGCaa--CUGCGggCUUaUGUCuGGC- -5'
26739 3' -52.2 NC_005808.1 + 7142 0.7 0.626575
Target:  5'- aGUCGUcGGCugcgGCCUGGAUGaccaGGGCCGg -3'
miRNA:   3'- gCAGCAaCUG----CGGGCUUAUg---UCUGGC- -5'
26739 3' -52.2 NC_005808.1 + 12447 0.75 0.358349
Target:  5'- uGcCGcUGAUGUCCGAcgcgGUGCAGGCCGg -3'
miRNA:   3'- gCaGCaACUGCGGGCU----UAUGUCUGGC- -5'
26739 3' -52.2 NC_005808.1 + 40228 0.66 0.855472
Target:  5'- --aCGcgGGCGUCUGccacGUGCAGGCCGa -3'
miRNA:   3'- gcaGCaaCUGCGGGCu---UAUGUCUGGC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.